Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.

Title data

Schreiber, Valerie ; Steegborn, Clemens ; Clausen, Tim ; Boos, Winfried ; Richet, Evelyne:
A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.
In: Molecular microbiology. Vol. 35 (February 2000) Issue 4 . - pp. 765-776.
ISSN 1365-2958
DOI: https://doi.org/10.1046/j.1365-2958.2000.01747.x

Abstract in another language

MalT, the transcriptional activator of the Escherichia coli maltose regulon, self-associates, binds promoter DNA and activates initiation of transcription only in the presence of ATP and maltotriose, the inducer. In vivo studies have revealed that MalT action is negatively controlled by the MalY protein. Using a biochemical approach, we analyse here the mechanism whereby MalY represses MalT activity. We show that MalY inhibits transcription activation by MalT in a purified transcription system. In vitro, a constitutive MalT variant (which is partially active in the absence of maltotriose) is less sensitive than wild-type MalT to repression by MalY, as observed in vivo. We demonstrate that MalY forms a complex with MalT only in the absence of maltotriose and that, conversely, MalY inhibits maltotriose binding by MalT. Together, these results establish that MalY acts directly upon MalT without the help of any factor, and that MalY is a negative effector of MalT competing with the inducer for MalT binding.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 10692154
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 13 Apr 2015 06:47
Last Modified: 10 Mar 2016 09:28
URI: https://eref.uni-bayreuth.de/id/eprint/10005