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Inhibition of the human deacylase Sirtuin 5 by the indole GW5074.

Title data

Suenkel, Benjamin ; Fischer, Frank ; Steegborn, Clemens:
Inhibition of the human deacylase Sirtuin 5 by the indole GW5074.
In: Bioorganic & Medicinal Chemistry Letters. Vol. 23 (1 January 2013) Issue 1 . - pp. 143-146.
ISSN 1464-3405
DOI: https://doi.org/10.1016/j.bmcl.2012.10.136

Abstract in another language

Sirtuins are NAD(+) consuming protein deacylases involved in many cellular processes from DNA-repair to metabolism. Their contribution to age-related and metabolic diseases makes them attractive pharmaceutical targets. Few pharmacological inhibitors have been reported yet for human Sirt5 since substrates and assays for reliable testing of its activity were unavailable until recently, and most modulators of other Sirtuins were not tested against Sirt5 and therefore have only partially characterized isoform selectivities. We used here improved substrates and assays for testing of known Sirtuin inhibitors for their effects on two activities of human Sirt5, the generic Sirtuin activity deacetylation and the more pronounced Sirt5 activity desuccinylation. Our tests show that most of the compounds have no significant effect on either Sirt5 activity. The indole GW5074, however, was found to be a potent inhibitor for Sirt5's desuccinylation activity, identifying a first pharmacological scaffold for development into Sirt5-specific inhibitors. Interestingly, the compound showed weaker effects in Sirt5 deacetylation assays and also varying potencies against different peptide sequences, indicating a substrate-specific effect of GW5074.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 23195732
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 17 Apr 2015 10:15
Last Modified: 17 Apr 2015 10:15
URI: https://eref.uni-bayreuth.de/id/eprint/10389