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Structural and energetic basis of infection by the filamentous bacteriophage IKe

Title data

Jakob, Roman P. ; Geitner, Anne-Juliane ; Weininger, Ulrich ; Balbach, Jochen ; Dobbek, Holger ; Schmid, Franz X.:
Structural and energetic basis of infection by the filamentous bacteriophage IKe.
In: Molecular microbiology. Vol. 84 (June 2012) Issue 6 . - pp. 1124-1138.
ISSN 1365-2958
DOI: https://doi.org/10.1111/j.1365-2958.2012.08079.x

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Abstract in another language

Filamentous phage use the two N-terminal domains of their gene-3-proteins to initiate infection of Escherichia coli. One domain interacts with a pilus, and then the other domain binds to TolA at the cell surface. In phage fd, these two domains are tightly associated with each other, which renders the phage robust but non-infectious, because the TolA binding site is inaccessible. Activation for infection requires partial unfolding, domain disassembly and prolyl isomerization. Phage IKe infects E. coli less efficiently than phage fd. Unlike in phage fd, the pilus- and TolA-binding domains of phage IKe are independent of each other in stability and folding. The site for TolA binding is thus always accessible, but the affinity is very low. The structures of the two domains, analysed by X-ray crystallography and by NMR spectroscopy, revealed a unique fold for the N-pilus-binding domain and a conserved fold for the TolA-binding domain. The absence of an activation mechanism as in phage fd and the low affinity for TolA probably explain the low infectivity of phage IKe. They also explain why, in a previous co-evolution experiment with a mixture of phage fd and phage IKe, all hybrid phage adopted the superior infection mechanism of phage fd.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 22591114
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 22 Apr 2015 07:54
Last Modified: 01 Mar 2016 10:12
URI: https://eref.uni-bayreuth.de/id/eprint/10542