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Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates

Title data

Schmid, Franz X.:
Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates.
In: FEBS Letters. Vol. 198 (31 March 1986) Issue 2 . - pp. 217-220.
ISSN 1873-3468
DOI: https://doi.org/10.1016/0014-5793(86)80408-2

Official URL: Volltext

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Abstract in another language

The trans----cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (tau = 500 s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10 degrees C. However, it is strongly accelerated (tau = 100 s) in samples which, prior to isomerization, had been converted to a folding intermediate by a 15 s refolding pulse under strongly native conditions (0.8 M ammonium sulfate, 0 degree C). The results demonstrate that extensive folding is possible before Pro 93 isomerizes to its native cis state and that the presence of structural folding intermediates leads to a marked increase in the rate of subsequent proline isomerization.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 3956730
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Professorship Biochemistry - Univ.-Prof. Dr. Franz Xaver Schmid
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 30 Apr 2015 12:01
Last Modified: 11 Mar 2016 10:45
URI: https://eref.uni-bayreuth.de/id/eprint/11228