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Elevated nicotianamine levels in Arabidopsis halleri roots play a key role in Zn hyperaccumulation

Title data

Deinlein, Ulrich ; Weber, Michael ; Schmidt, Holger ; Rensch, Stefan ; Trampczynska, Aleksandra ; Hansen, Thomas H. ; Husted, Søren ; Schjørring, Jan K. ; Talke, Ina N. ; Krämer, Ute ; Clemens, Stephan:
Elevated nicotianamine levels in Arabidopsis halleri roots play a key role in Zn hyperaccumulation.
In: The Plant Cell. Vol. 24 (2012) Issue 2 . - pp. 708-723.
ISSN 1532-298X
DOI: https://doi.org/10.1105/tpc.111.095000

Abstract in another language

Zinc deficiency is among the leading health risk factors in developing countries. Breeding of Zn-enriched crops is expected to be facilitated by molecular dissection of plant Zn hyperaccumulation, i.e. the ability of certain plants to accumulate Zn to levels > 100-fold higher than normal plants. The model hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to non-accumulators among a core of alterations in metal homeostasis. Suppression of AhNAS2 by RNAi resulted in strongly reduced root nicotianamine (NA) accumulation and a concomitant decrease in root-to-shoot-translocation of Zn. Speciation analysis by size-exclusion chromatography coupled to inductively coupled plasma mass spectrometry showed that the dominating Zn-ligands in roots were nicotianamine and thiols. In AhNAS2-RNAi plants a marked increase in Zn-thiol species was observed. Wild-type A. halleri cultivated on their native soil showed elemental profiles very similar to those found in field samples. Leaf Zn concentrations in AhNAS2-RNAi lines, however, did not reach the Zn hyperaccumulation threshold. Leaf Cd accumulation was also significantly reduced. These results demonstrate a role for AhNAS2 in Zn hyperaccumulation also under near-natural conditions. We propose that NA forms complexes with Zn(II) in root cells and facilitates symplastic passage of Zn(II) towards the xylem.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER104736
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Apr 2015 15:42
Last Modified: 12 Feb 2019 09:57
URI: https://eref.uni-bayreuth.de/id/eprint/11643