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Novel oligonucleotide probes for in situ detection of pederin-producing endosymbionts of Paederus riparius rove beetles (Coleoptera: Staphylinidae)

Title data

Kador, Matthias ; Horn, Marcus A. ; Dettner, Konrad:
Novel oligonucleotide probes for in situ detection of pederin-producing endosymbionts of Paederus riparius rove beetles (Coleoptera: Staphylinidae).
In: FEMS Microbiology Letters. Vol. 319 (2011) Issue 1 . - pp. 73-81.
ISSN 1574-6968
DOI: https://doi.org/10.1111/j.1574-6968.2011.02270.x

Abstract in another language

Bacterial endosymbionts from female Paederus rove beetles are hitherto uncultured, phylogenetically related to Pseudomonas sp., and produce the polyketide pederin, which exhibits strong cytotoxic effects and antitumoral activities. The location of such endosymbionts inside beetles and on beetles' eggs is hypothesized based on indirect evidence rather than elucidated. Thus, an endosymbiont-specific and a competitor oligonucleotide-probe (Cy3-labelled PAE444 and unlabelled cPAE444, respectively) were designed and utilized for fluorescence in situ hybridization (FISH) with semi thin-sections of Paederus riparius eggs. Cy3-PAE444 positive cells were densely packed and covered the whole eggshell. 100% of EUB338-Mix-positive total bacterial cells were PAE444 positive, indicating a biofilm dominated by Paederus endosymbionts. Analysis of different egg deposition stadiums by electron microscopy and pks (polyketide synthase gene, a structural gene associated with pederin biosynthesis)-PCR supported results obtained by FISH and revealed that the endosymbiont-containing layer is applied to the eggshell inside the efferent duct. These findings suggest that P. riparius endosymbionts are located inside unknown structures of the female genitalia, which allow for a well regulated release of endosymbionts during oviposition. The novel oligonucleotide probes developed in this study will facilitate (i) the identification of symbiont-containing structures within genitalia of their beetle hosts and (ii) directed cultivation approaches in the future.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER97333
BAYCEER103064
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology II - Evolutionary Animal Ecology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Former Professors > Chair Animal Ecology II - Univ.-Prof. Dr. Konrad Dettner
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Apr 2015 15:42
Last Modified: 29 Jul 2015 09:44
URI: https://eref.uni-bayreuth.de/id/eprint/11693