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Effect of sulfide on the cytotoxicity of arsenite and arsenate in human hepatocytes (HepG2) and human urothelial cells (UROtsa)

Title data

Hinrichsen, Sinikka ; Lohmayer, Regina ; Zdrenka, Ricarda ; Dopp, Elke ; Planer-Friedrich, Britta:
Effect of sulfide on the cytotoxicity of arsenite and arsenate in human hepatocytes (HepG2) and human urothelial cells (UROtsa).
In: Environmental Science and Pollution Research. Vol. 121 (2014) Issue 17 . - pp. 10151-10162.
ISSN 1614-7499
DOI: https://doi.org/10.1007/s11356-014-2950-4

Abstract in another language

Arsenic, a common poison, is known to react with sulfide in vivo, forming thioarsenates. The acute toxicity of the inorganic thioarsenates is currently unknown. Our experiments showed that a fourfold sulfide excess reduced acute arsenite cytotoxicity in human hepatocytes (HepG2) and urothelial cells (UROtsa) significantly, but had little effect on arsenate toxicity. Speciation analysis showed immediate formation of thioarsenates (up to 73 % of total arsenic) in case of arsenite, but no speciation changes for arsenate. Testing acute toxicity of mono- and trithioarsenate individually, both thioarsenates were found to be more toxic than their structural analogue arsenate, but less toxic than arsenite. Toxicity increased with the number of thio-groups. The amount of cellular arsenic uptake after 24 h corresponded to the order of toxicity of the four compounds tested. The dominant to almost exclusive intracellular arsenic species was arsenite. The results imply that thiolation is a detoxification process for arsenite in sulfidic milieus. The mechanism could either be that thioarsenates regulate the amount of free arsenite available for cellular uptake without entering the cells themselves, or, based on their chemical similarity to arsenate, they could be taken up by similar transporters and reduced rapidly intracellularly to arsenite.

Further data

Item Type: Article in a journal
Refereed: No
Additional notes: BAYCEER122540
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Professorship Environmental Geochemistry Group
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Professorship Environmental Geochemistry Group > Professorship Environmental Geochemistry - Univ.-Prof. Dr. Britta Planer-Friedrich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 03 May 2015 08:56
Last Modified: 03 May 2015 08:56
URI: https://eref.uni-bayreuth.de/id/eprint/12618