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A structural model for the full-length blue light-sensing protein YtvA from Bacillus subtilis, based on EPR spectroscopy

Title data

Engelhard, Christopher ; Raffelberg, Sarah ; Tang, Yifen ; Diensthuber, Ralph P. ; Möglich, Andreas ; Losi, Aba ; Gärtner, Wolfgang ; Bittl, Robert:
A structural model for the full-length blue light-sensing protein YtvA from Bacillus subtilis, based on EPR spectroscopy.
In: Photochemical & Photobiological Sciences. Vol. 12 (October 2013) Issue 10 . - pp. 1855-1863.
ISSN 1474-9092
DOI: https://doi.org/10.1039/c3pp50128k

Project information

Project financing: Alexander von Humboldt-Stiftung
DFG

Abstract in another language

A model for the full-length structure of the blue light-sensing protein YtvA from Bacillus subtilis has been determined by EPR spectroscopy, performed on spin labels selectively inserted at amino acid positions 54, 80, 117 and 179. Our data indicate that YtvA forms a dimer in solution and enable us, based on the known structures of the individual domains and modelling, to propose a three-dimensional model for the full length protein. Most importantly, this includes the YtvA N-terminus that has so far not been identified in any structural model. We show that our data are in agreement with the crystal structure of an engineered LOV-domain protein, YF1, that shows the N-terminus of the protein to be helical and to fold back in between the β-sheets of the two LOV domains, and argue for an identical arrangement in YtvA. While we could not detect any structural changes upon blue-light activation of the protein, this structural model now forms an ideal basis for identifying residues as targets for further spin labelling studies to detect potential conformational changes upon irradiation of the protein.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 23900620
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 19 May 2015 07:07
Last Modified: 19 May 2015 07:07
URI: https://eref.uni-bayreuth.de/id/eprint/13596