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Determination of residual dipolar couplings in homonuclear MOCCA-SIAM experiments

Title data

Möglich, Andreas ; Wenzler, Michael ; Kramer, Frank ; Glaser, Steffen J. ; Brunner, Eike:
Determination of residual dipolar couplings in homonuclear MOCCA-SIAM experiments.
In: Journal of Biomolecular NMR. Vol. 23 (July 2002) Issue 3 . - pp. 211-219.
ISSN 1573-5001
DOI: https://doi.org/10.1023/A:1019841602348

Abstract in another language

In solutions with partial molecular alignment, anisotropic magnetic interactions such as the chemical shift anisotropy, the electric quadrupole interaction, and the magnetic dipole-dipole interaction are no longer averaged out to zero in contrast to isotropic solutions. The resulting residual anisotropic magnetic interactions are increasingly used in biological NMR studies for the determination of 3D structures of proteins and other biomolecules. In the present paper we propose a new approach allowing the measurement of residual HN-H(alpha) dipolar couplings of non-isotope enriched proteins based on the application of the MOCCA-SIAM experiment. This experiment allows the measurement of homonuclear coupling constants with an accuracy of ca. +/- 0.2 Hz and is therefore particularly well suited to determine residual dipolar couplings at relatively low degrees of molecular orientation. The agreement between experimentally determined residual HN-H(alpha) couplings and calculated values is demonstrated for BPTI.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PubMed-ID: 12238593
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
Date Deposited: 20 May 2015 08:51
Last Modified: 20 May 2015 08:51
URI: https://eref.uni-bayreuth.de/id/eprint/13620