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Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins

Title data

Lang, Gregor ; Jokisch, Stephan ; Scheibel, Thomas:
Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins.
In: Journal of Visualized Experiments. (8 May 2013) Issue 75 . - p. 50492. - 10 Seiten.
ISSN 1940-087X
DOI: https://doi.org/10.3791/50492

Official URL: Volltext

Abstract in another language

Based on the natural sequence of Araneus diadematus Fibroin 4 (ADF4), the recombinant spider silk protein eADF4(C16) has been engineered.
This highly repetitive protein has a molecular weight of 48kDa and is soluble in different solvents (hexafluoroisopropanol (HFIP), formic acid and
aqueous buffers). eADF4(C16) provides a high potential for various technical applications when processed into morphologies such as films,
capsules, particles, hydrogels, coatings, fibers and nonwoven meshes. Due to their chemical stability and controlled morphology, the latter can
be used to improve filter materials. In this protocol, we present a procedure to enhance the efficiency of different air filter devices, by deposition
of nonwoven meshes of electrospun recombinant spider silk proteins. Electrospinning of eADF4(C16) dissolved in HFIP results in smooth fibers.
Variation of the protein concentration (5-25% w/v) results in different fiber diameters (80-1,100 nm) and thus pore sizes of the nonwoven mesh.
Post-treatment of eADF4(C16) electrospun from HFIP is necessary since the protein displays a predominantly α-helical secondary structure in
freshly spun fibers, and therefore the fibers are water soluble. Subsequent treatment with ethanol vapor induces formation of water resistant,
stable β-sheet structures, preserving the morphology of the silk fibers and meshes. Secondary structure analysis was performed using Fourier
transform infrared spectroscopy (FTIR) and subsequent Fourier self-deconvolution (FSD).
The primary goal was to improve the filter efficiency of existing filter substrates by adding silk nonwoven layers on top. To evaluate the influence
of electrospinning duration and thus nonwoven layer thickness on the filter efficiency, we performed air permeability tests in combination with
particle deposition measurements. The experiments were carried out according to standard protocols.
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Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Bioengineering; Biochemistry; Chemistry; Materials Science; Molecular Biology; Cellular Biology; Proteins; Nanotechnology;
materials (general); materials handling; nanodevices (mechanical); structural analysis; spider silk; electrospinning; microfibers; nonwoven; filter;
mesh; biomaterials
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Faculties > Faculty of Engineering Science > Chair Biomaterials
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 19 Jun 2015 06:48
Last Modified: 26 Nov 2015 10:51
URI: https://eref.uni-bayreuth.de/id/eprint/15287