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Advantages of compound-specific stable isotope measurements over bulk measurements in studies on plant uptake of intact amino acids

Title data

Sauheitl, Leopold ; Glaser, Bruno ; Weigelt, Alexandra:
Advantages of compound-specific stable isotope measurements over bulk measurements in studies on plant uptake of intact amino acids.
In: Rapid Communications in Mass Spectrometry. Vol. 23 (2009) Issue 20 . - pp. 3333-3342.
ISSN 0951-4198
DOI: https://doi.org/10.1002/rcm.4255

Abstract in another language

Increasing interest in the ability of plants to take up amino acids has given rise to questions on theaccuracy of the commonly used bulk method to measure and calculate amino acid uptake. Thismethod uses bulk measurements of 13C and 15N enrichment in plant tissues after application of duallabelledamino acids but some authors have recommended the use of compound-specific stableisotope (CSI) analysis of the plants’ amino acids instead. However, there has never been a directevaluation of both methods.We conducted a field study applying dual-labelled (13C, 15N) amino acids(glycine, valine, tyrosine and lysine) to soil of a Plantago lanceolata monoculture. Root and shootsamples were collected 24 h after label application and the isotope composition of the plant tissueswas investigated using bulk and CSI measurements. Enrichment of 13C in the case of CSI measurementswas limited to the applied amino acids, showing that no additional 13C had been incorporatedinto the plants’ amino acid pool via the uptake of tracer-derived C-fragments. Compared with thisrather conservative indicator of amino acid uptake, the 13C enrichment of bulk measurements was 8,5, 1.6 and 6 times higher for fine roots, storage roots, shoot and the whole plant, respectively. Thesefindings show that the additional uptake of tracer-derived C-fragments will result in a considerableoverestimation of amino acid uptake in the case of bulk measurements. We therefore highlyrecommend the use of CSI measurements for future amino acid uptake studies due to their higheraccuracy. Copyright # 2009 John Wiley & Sons, Ltd.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER76513
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Soil Physics
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 06 Jul 2015 10:25
Last Modified: 06 Jul 2015 10:25
URI: https://eref.uni-bayreuth.de/id/eprint/15908