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Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil

Title data

Peršoh, Derek ; Theuerl, Susanne ; Buscot, François ; Rambold, Gerhard:
Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil.
In: Journal of Microbiological Methods. Vol. 75 (2008) Issue 1 . - pp. 19-24.
ISSN 0167-7012
DOI: https://doi.org/10.1016/j.mimet.2008.04.009

Abstract in another language

[PubMed]A universally adaptable protocol for quantitative extraction of high-purity nucleic acids from soil is presented. A major problem regarding the extraction of nucleic acids from soil is the presence of humic substances, which interfere with the extraction process itself and in subsequent analytical manipulations. By the approach described here, the humic compounds are precipitated prior to cell lysis with Al2(SO4)3, and thus eliminated prior to the nucleic acid extraction. The protocol allows for removing of a considerable content and range of humic acids and should therefore be applicable for a wide spectrum of soil types. Accordingly, reproducible results in analyses of different soil types are made possible, inclusively for quantitative comparisons.

Further data

Item Type: Article in a journal
Refereed: No
Additional notes: BAYCEER60961
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Mycology
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 14 Jul 2015 06:21
Last Modified: 26 Jul 2017 07:15
URI: https://eref.uni-bayreuth.de/id/eprint/16347