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Different atmospheric methane-oxidizing communities in European beech and Norway spruce soils

Title data

Degelmann, Daniela M. ; Borken, Werner ; Drake, Harold L. ; Kolb, Steffen:
Different atmospheric methane-oxidizing communities in European beech and Norway spruce soils.
In: Applied and Environmental Microbiology. Vol. 76 (2010) Issue 10 . - pp. 3228-3235.
ISSN 1098-5336
DOI: https://doi.org/10.1128/AEM.02730-09

Abstract in another language

Norway spruce (Picea abies) forests exhibit lower annual atmospheric methane consumption rates than do European beech (Fagus sylvatica) forests. In the current study, pmoA (encodes a subunit of membrane-bound CH4 monooxygenase) genes from three temperate forest ecosystems with both beech and spruce stands were analyzed to assess the potential effect of tree species on methanotrophic communities. A pmoA sequence difference of 7% at the derived protein level correlated with the species-level distance cut-off value of 3% based on the 16S rRNA gene. Applying this distance cut-off, higher numbers of species-level pmoA genotypes were detected in beech than in spruce soil samples, all affiliating with upland soil cluster  (USC). Additionally, two deep branching genotypes (named 6 and 7) were present in various soil samples not affiliating with both pmoA or amoA. Abundance of USCα pmoA genes was higher in beech soils and reached up to (1.2 ± 0.2) ×108 pmoA genes gDW-1. Calculated atmospheric methane oxidation rates per cell yielded the same trend. However, these values were below the theoretical threshold necessary for facilitating cell maintenance, suggesting that USCα species might require alternative carbon or energy sources to thrive in forest soils. These collective results indicate that the methanotrophic diversity and abundance in spruce soils are lower than those of beech soils, suggesting that tree species-related factors might influence the in situ activity of methanotrophs.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER80835
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology > Chair Ecological Microbiology - Univ.-Prof. Harold L. Drake, Ph.D.
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Soil Ecology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 11 Sep 2015 06:32
Last Modified: 11 Sep 2015 06:32
URI: https://eref.uni-bayreuth.de/id/eprint/19078