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Reversible closing of water channels in Chara internodes provides evidence for a composite transport model of the plasma membrane

Title data

Henzler, Tobias ; Steudle, Ernst:
Reversible closing of water channels in Chara internodes provides evidence for a composite transport model of the plasma membrane.
In: Journal of Experimental Botany. Vol. 46 (1995) Issue 2 . - pp. 199-209.
ISSN 1460-2431
DOI: https://doi.org/10.1093/jxb/46.2.199

Abstract in another language

Treatment of internodal cells of Chara corallina with the water channel blocker HgCl2 caused a decrease of the hydraulic conductivity of the membrane (Lp) by a factor of three to four, In the presence of (practically) non-permeating solutes such as sugars or salts, the osmotic responses were similar to those found in controls, i.e. reflection coefficients (sigma(s)) were close to unity. However, when treating the internodes with osmotic solutions of rapidly permeating lipophilic substances such as low molecular weight alcohols or acetone, the picture of biphasic pressure relaxations due to the exchange of both water and solutes changed considerably. In the presence of HgCl2, reflection coefficients were substantially reduced and even became negative for some solutes (anomalous osmosis). Different from reflection coefficients, permeability coefficients (P-s) remained constant upon treatment, When using heavy water (HDO) as a small hydrophilic solute which should cross the membrane largely via water channels, results were different: the reflection coefficient of HDO increased and permeability decreased. Treating the cells with 5 mM 2-mercaptoethanol to remove the mercury from transport proteins (water channels) reversed changes in Lp and sigma(s). The results disagree with conventional membrane models, which imply a homogeneous membrane, They are readily explained by a composite model of the membrane in which proteinaceous arrays with specific water channels are arranged in parallel with lipid arrays. The latter account for most of the permeability of the small organic test solutes used and are not affected by the channel blocker. From the data, detailed information is obtained about transport properties of the arrays (water channels).

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER33682
Institutions of the University: Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Ecology
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 11 Sep 2015 06:35
Last Modified: 11 Sep 2015 06:35
URI: https://eref.uni-bayreuth.de/id/eprint/19406