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Microenvironments and mass transfer phenomena in biofilms studied with microsensors

Titelangaben

de Beer, Dirk ; Schramm, Andreas:
Microenvironments and mass transfer phenomena in biofilms studied with microsensors.
In: Rittmann, Bruce E. (Hrsg.): Microbial ecology of biofilms : Selected Proceedings of the International Specialty Conference on Microbial Ecology of Biofilms : Concepts, Tools and Applications. - Oxford : Pergamon , 1999 . - S. 173-178 . - (Water Science and Technology ; 39,7 )
ISBN 0-08-043654-4
DOI: https://doi.org/10.1016/S0273-1223(99)00165-1

Abstract

Direct observations on chemical microenvironment and microbial composition in biofilms are rare. The combination of microsensor- and molecular techniques is highly usefull for studies on the microbial ecology of biofilms. We shortly describe some applications of microsensors to study mass transfer phenomena and microbial processes in biofilms. It has recent been recognized that biofilms are not always flat layers of cells, but can consist of complex structures allowing liquid flow. Thus the classical view, that transport in biofilms is diffusional, is challenged. In laboratory grown biofilms the effect of convection on mass transfer was demonstrated. The microsensor technique has improved, so that direct in situ measurements in living biofilms are possible, also in bioreactors. We show hat also biofilms grown in bioreactors heterogeneity and convectional transport must be taken into account. For the description of the microbial population we use molecular techniques, such as in situ hybridisation with 16S-rRNA probes. In a nitrifying/denitrifying biofilm we found a nitrifying population that was much more complex as normally found with cultivation studies. It has become clear that the main nitrifying species found by cultivation techniques (Nitrosomonas and Nitrobacter) are minor populations in wild-type biofilms, which are dominated by Nitrospira and Nitrosospira strains.

Weitere Angaben

Publikationsform: Aufsatz in einem Buch
Begutachteter Beitrag: Ja
Zusätzliche Informationen: BAYCEER8867
Institutionen der Universität: Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Ökologische Mikrobiologie
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie
Forschungseinrichtungen
Forschungseinrichtungen > Forschungszentren
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
Eingestellt am: 30 Sep 2015 05:56
Letzte Änderung: 30 Sep 2015 05:56
URI: https://eref.uni-bayreuth.de/id/eprint/19946