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Investigation and molecular mimicry of the antigen involved in the interaction between the monoclonal antibody 5D10 and the human breast cancer cell line MCF-7

Title data

Vandevyver, Caroline ; Canarelli, Stéphane ; Bossen, Claudia ; Fisch, Igor ; Motmans, Kris ; Raus, Jef ; Freitag, Ruth:
Investigation and molecular mimicry of the antigen involved in the interaction between the monoclonal antibody 5D10 and the human breast cancer cell line MCF-7.
In: Biotechnology and Bioengineering. Vol. 97 (July 2007) Issue 4 . - pp. 721-734.
ISSN 1097-0290
DOI: https://doi.org/10.1002/bit.21276

Official URL: Volltext

Abstract in another language

Monoclonal antibody (mAb) 5D10 is directed against the human breast cancer cell line MCF-7. Biochemical characterization of the antibody epitope was attempted and revealed a complex, most likely carbohydrate-linked nature, which prevented isolation and further studies of the interaction. A major goal of this work was to generate structural mimics of the 5D10 epitope to serve as putative substitutes in such studies. A peptide library displayed on filamentous phage was used to select for mimotope peptide sequences. All positive phage clones selected from the library displayed the amino acid sequence H(2)N-QMNPMYYR-CO(2)H. This peptide sequence, as well as a branched form of the peptide, was found to bind mAb 5D10. Moreover, both peptide sequences were able to inhibit the binding of 5D10 to the MCF-7 cells in a concentration-dependent manner, with an EC(50) value in the range of 65 microM. According to these results, random phage peptide libraries can serve to identify mimotopic peptides for unknown complex cell surface epitopes.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: antibody, antigen mimicry, cancer, carbohydrate antigen, mimotope, peptide library, phage-display
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: No
DDC Subjects: 500 Science
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 24 Feb 2016 15:41
Last Modified: 24 Feb 2016 15:41
URI: https://eref.uni-bayreuth.de/id/eprint/31047