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Influence of Polyplex Formation on the Performance of Star-Shaped Polycationic Transfection Agents for Mammalian Cells

Titelangaben

Raup, Alexander ; Stahlschmidt, Ullrich ; Jérôme, Valérie ; Synatschke, Christopher V. ; Müller, Axel H. E. ; Freitag, Ruth:
Influence of Polyplex Formation on the Performance of Star-Shaped Polycationic Transfection Agents for Mammalian Cells.
Process Biotechnology, University of Bayreuth, 95440 Bayreuth, Germany; Simpson Querrey Institute for BioNanotechnology, Northwestern University, Chicago, 60611 IL, USA; Institute of Organic Chemistry, Johannes-Gutenberg-University, 55099 Mainz, Germany
In: Polymers. Bd. 8 (Juni 2016) Heft 6 . - S. 224.
ISSN 2073-4360
DOI: https://doi.org/10.3390/polym8060224

Volltext

Link zum Volltext (externe URL): Volltext

Abstract

Genetic modification (“transfection”) of mammalian cells using non-viral, synthetic agents
such as polycations, is still a challenge. Polyplex formation between the DNA and the polycation
is a decisive step in such experiments. Star-shaped polycations have been proposed as superior
transfection agents, yet have never before been compared side-by-side, e.g., in view of structural
effects. Herein four star-shaped polycationic structures, all based on (2-dimethylamino) ethyl
methacrylate (DMAEMA) building blocks, were investigated for their potential to deliver DNA
to adherent (CHO, L929, HEK-293) and non-adherent (Jurkat, primary human T lymphocytes)
mammalian cells. The investigated vectors included three structures where the PDMAEMA arms
(different arm length and grafting densities) had been grown from a center silsesquioxane or
silica-coated γ-Fe2O3-core and one micellar structure self-assembled from poly(1,2-butadiene)-block
PDMAEMA polymers. All nano-stars combined high transfection potential with excellent
biocompatibility. The micelles slightly outperformed the covalently linked agents. For method
development and optimization, the absolute amount of polycation added to the cells was more
important than the N/P-ratio (ratio between polycation nitrogen and DNA phosphate), provided a
lower limit was passed and enough polycation was present to overcompensate the negative charge
of the plasmid DNA. Finally, the matrix (NaCl vs. HEPES-buffered glucose solution), but also the
concentrations adjusted during polyplex formation, affected the results.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: gene delivery; mammalian cells; non-viral; PDMAEMA; T lymphocytes; transfection
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 07 Jun 2016 08:28
Letzte Änderung: 07 Jun 2016 08:28
URI: https://eref.uni-bayreuth.de/id/eprint/32539