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Electrochemistry of laccase at multi-walled carbon nanotube modified electrodes : investigation of various immobilisation conditions and electrode configurations

Title data

Hämmerle, Martin ; Hilgert, Karin ; Moos, Ralf:
Electrochemistry of laccase at multi-walled carbon nanotube modified electrodes : investigation of various immobilisation conditions and electrode configurations.
2016
Event: Biosensors 2016, 26th Anniversary World Congress on Biosensors , May 25.-27. 2016 , Gothenburg, Sweden.
(Conference item: Conference , Poster )

Abstract in another language

In enzymatic biofuel cells the efficient electron transfer from the enzymes to the electrodes plays a key role. There are two possibilities: mediated or direct electron transfer. Whereas mediated electron transfer is well established, the use of mediators may also induce problems: instability of the mediator, and reduced attainable cell voltage. Thus, there is interest in direct electron transfer, although this approach often suffers from lower current and power densities. In enzymatic biofuel cells multi-copper oxidases such as bilirubin oxidase or laccase are widely used for the cathode, where they catalyse the oxygen reduction reaction to water. For laccase direct electron transfer was observed on nanostructured materials such as nanoporous gold or carbon nanotubes.

The focus of the presented work is the electrochemistry of laccase without mediator at multi-walled carbon nanotube (MWCNT) modified electrodes. Various commercial laccases from different organisms are used. Laccase is immobilised on MWCNT by adsorption without linker in order to achieve a small distance between enzyme and electrode, and to support direct electron transfer. Various immobilisation conditions are investigated: pH, type of buffer, pre-treatment of MWCNT. Either laccase is first adsorbed on MWCNT in solution and then attached onto the electrode, or the electrode is first modified with MWCNT and then laccase is adsorbed. MWCNT itself is either adsorbed on the electrode surface, or entrapped within a polymer, or retained with a membrane. The electrochemistry of the laccase/MWCNT modified electrodes is investigated e.g. by cyclic voltammetry under air, oxygen, or nitrogen.

As oxygen concentration in water is low also gas diffusion electrodes are explored. Mixing the laccase modified MWCNT with PTFE (Polytetrafluoroethylene) on conducting supports various electrode configurations are tested. Thus, three-phase-boundaries are created for efficient mass transport of oxygen to the electrode in the gas phase avoiding the problem of limited oxygen solubility in water.

Further data

Item Type: Conference item (Poster)
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos
Profile Fields > Advanced Fields > Advanced Materials
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Faculties
Faculties > Faculty of Engineering Science > Chair Functional Materials
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 08 Jun 2016 08:57
Last Modified: 08 Jun 2016 08:57
URI: https://eref.uni-bayreuth.de/id/eprint/32553