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Cations influence the cross-linking of hydrogels made of recombinant, polyanionic spider silk proteins

Title data

DeSimone, Elise ; Schacht, Kristin ; Scheibel, Thomas:
Cations influence the cross-linking of hydrogels made of recombinant, polyanionic spider silk proteins.
In: Materials Letters. Vol. 183 (15 November 2016) . - pp. 101-104.
ISSN 0167-577X
DOI: https://doi.org/10.1016/j.matlet.2016.07.044

Abstract in another language

Hydrogels made of polyanionic recombinant spider silk proteins (spidroins) were prepared either in the presence or the absence of Dulbecco's Modified Eagle Medium (DMEM). Mono- and divalent cations present in DMEM severely affected the self-assembly process of the spidroins. Although the addition of DMEM had no apparent effect on secondary structure formation, there was a significant effect on the kinetics as well as on the hydrogel network; in the presence of DMEM, gelation occurred more rapidly. Additionally, the hydrogels were stiffer; however, the hydrogels were still shear-thinning. In summary, it can be concluded that there is a significant impact of ionic cross-linking on recombinant spidroin-based hydrogels.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Bioinks; Biofabrication; 3D Bioprinting; Recombinant spider silk protein; Physical crosslinking; Spidroin
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Emerging Fields
Profile Fields > Emerging Fields > Food and Health Sciences
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 27 Jul 2016 13:06
Last Modified: 27 Jul 2016 13:06
URI: https://eref.uni-bayreuth.de/id/eprint/33666