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Cytotoxic activity of selenosulfate versus selenite in tumor cells depends on cell line and presence of amino acids

Title data

Hinrichsen, Sinikka ; Planer-Friedrich, Britta:
Cytotoxic activity of selenosulfate versus selenite in tumor cells depends on cell line and presence of amino acids.
In: Environmental Science and Pollution Research. Vol. 23 (2016) Issue 9 . - pp. 8349-8357.
ISSN 1614-7499
DOI: https://doi.org/10.1007/s11356-015-5960-y

Abstract in another language

Based on acute cytotoxicity studies, selenosulfate (SeSO3-) has been suggested to possess a generally higher toxic activity in tumor cells than selenite. The reason for this difference in cytotoxic activity remained unclear. In the present study, cytotoxicity tests with human hepatoma (HepG2), malignant melanoma (A375), and urinary bladder carcinoma cells (T24) showed that the selenosulfate toxicity was very similar between all three tested cell lines (IC50 6.6-7.1 µM after 24 h). It was largely independent of exposure time and presence or absence of amino acids. What changed, however, was the toxicity of selenite, which was lower than that of selenosulfate only for HepG2 cells (IC50 > 15 µM), but similar to and higher than that of selenosulfate for A375 (IC50 4.7 µM) and T24 cells (IC50 3.5 µM), respectively. Addition of amino acids to T24 cell growth medium down-regulated short-term selenite uptake (1.5 versus 12.9 ng Se /106 cells) and decreased its cytotoxicity (IC50 8.4 µM), rendering it less toxic than selenosulfate. The suggested mechanism is a stronger expression of the xc-¬ transport system in the more sensitive T24 compared to HepG2 cells which creates a reductive extracellular microenvironment and facilitates selenite uptake by reduction. Selenosulfate is already reduced and so less affected. The cytotoxic activity of selenosulfate and selenite to tumor cells therefore depends on the sensitivity of each cell line, supplements like amino acids as well as the reductive state of the extracellular environment.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER133103
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Professorship Environmental Geochemistry Group
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Professorship Environmental Geochemistry Group > Professorship Environmental Geochemistry - Univ.-Prof. Dr. Britta Planer-Friedrich
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 11 Aug 2016 07:45
Last Modified: 11 Aug 2016 07:45
URI: https://eref.uni-bayreuth.de/id/eprint/33888