Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Transcription is regulated by NusA:NusG interaction

Title data

Strauß, Martin ; Vitiello, Christal ; Schweimer, Kristian ; Gottesman, Max E. ; Rösch, Paul ; Knauer, Stefan H.:
Transcription is regulated by NusA:NusG interaction.
In: Nucleic Acids Research. Vol. 44 (2016) Issue 12 . - pp. 5971-5982.
ISSN 1362-4962
DOI: https://doi.org/10.1093/nar/gkw423

Abstract in another language

NusA and NusG are major regulators of bacterial transcription elongation, which act either in concert or antagonistically. Both bind to RNA polymerase (RNAP), regulating pausing as well as intrinsic and Rho-dependent termination. Here, we demonstrate by nuclear magnetic resonance spectroscopy that the Escherichia coli NusG amino-terminal domain forms a complex with the acidic repeat domain 2 (AR2) of NusA. The interaction surface of either transcription factor overlaps with the respective binding site for RNAP. We show that NusA-AR2 is able to remove NusG from RNAP. Our in vivo and in vitro results suggest that interaction between NusA and NusG could play various regulatory roles during transcription, including recruitment of NusG to RNAP, resynchronization of transcription:translation coupling, and modulation of termination efficiency.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Profile Fields > Advanced Fields > Polymer and Colloid Science
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Profile Fields
Profile Fields > Advanced Fields
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 08 Aug 2016 08:50
Last Modified: 01 Feb 2018 08:11
URI: https://eref.uni-bayreuth.de/id/eprint/34072