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Resonance assignment of an engineered amino-terminal domain of a major ampullate spider silk with neutralized charge cluster

Title data

Schaal, Daniel ; Bauer, Joschka ; Schweimer, Kristian ; Scheibel, Thomas ; Rösch, Paul ; Schwarzinger, Stephan:
Resonance assignment of an engineered amino-terminal domain of a major ampullate spider silk with neutralized charge cluster.
In: Biomolecular NMR Assignments. Vol. 10 (2016) Issue 1 . - pp. 199-202.
ISSN 1874-270X
DOI: https://doi.org/10.1007/s12104-016-9666-y

Abstract in another language

Spider dragline fibers are predominantly made out of the major ampullate spidroins (MaSp) 1 and 2. The assembly of dissolved spidroin into a stable fiber is highly controlled for example by dimerization of its amino-terminal domain (NRN) upon acidification, as well as removal of sodium chloride along the spinning duct. Clustered residues D39, E76 and E81 are the most highly conserved residues of the five-helix bundle, and they are hypothesized to be key residues for switching between a monomeric and a dimeric conformation. Simultaneous replacement of these residues by their non-titratable analogues results in variant D39N/E76Q/E81Q, which is supposed to fold into an intermediate conformation between that of the monomeric and the dimeric state at neutral pH. Here we report the resonance assignment of Latrodectus hesperus NRN variant D39N/E76Q/E81Q at pH 7.2 obtained by high-resolution triple resonance NMR spectroscopy.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Research Institutions > Research Centres > Research Center for Bio-Macromolecules - BIOmac
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Profile Fields
Research Institutions
Research Institutions > Research Centres
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 08 Aug 2016 09:00
Last Modified: 08 Aug 2016 09:00
URI: https://eref.uni-bayreuth.de/id/eprint/34073