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Recombinant Production, Characterization, and Fiber Spinning of an Engineered Short Major Ampullate Spidroin (MaSp1s)

Titelangaben

Thamm, Christopher ; Scheibel, Thomas:
Recombinant Production, Characterization, and Fiber Spinning of an Engineered Short Major Ampullate Spidroin (MaSp1s).
In: Biomacromolecules. (24 Februar 2017) .
ISSN 1526-4602
DOI: 10.1021/acs.biomac.7b00090

Abstract

Spider dragline silk exhibits an extraordinary toughness and is typically composed of two types of major ampullate spidroins (MaSp1 and MaSp2), differing in their proline content and hydrophobicity. In this paper, we recombinantly produced an unusual but naturally occurring short major ampullate spidroin (MaSp1s) as a fusion construct between established Latrodectus hesperus terminal domains and the novel Cyrtophora moluccensis core domain. The sequence of the recombinant spidroin was engineered to guarantee high yields upon recombinant production and was named eMaSp1s. Its solution structure as well as the mechanical properties of wet-spun eMaSp1s fibers were examined. Structural characterization using CD- and FTIR spectroscopy showed a predominantly α-helical solution structure and a high ß-sheet content within fibers. Surprisingly, eMaSp1s fibers show similar mechanical properties as wet-spun fibers of other engineered spider silk proteins, albeit eMaSp1s has a lower molecular weight and not the typical sequence repeats in its core domain. Therefore, the findings provide insights into the molecular interplay necessary to obtain the typical silk fiber mechanics.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien > Lehrstuhl Biomaterialien - Univ.-Prof. Dr. Thomas Scheibel
Profilfelder
Profilfelder > Advanced Fields
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Profilfelder > Advanced Fields > Neue Materialien
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Profilfelder > Emerging Fields
Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften
Forschungseinrichtungen
Forschungseinrichtungen > Forschungszentren
Forschungseinrichtungen > Forschungszentren > Bayreuther Materialzentrum - BayMAT
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 24 Mär 2017 08:27
Letzte Änderung: 24 Mär 2017 08:27
URI: https://eref.uni-bayreuth.de/id/eprint/36627