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Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor

Title data

Berntsson, Oskar ; Diensthuber, Ralph P. ; Panman, Matthijs R. ; Björling, Alexander ; Hughes, Ashley J. ; Henry, Léocadie ; Niebling, Stephan ; Newby, Gemma ; Liebi, Marianne ; Menzel, Andreas ; Henning, Robert ; Kosheleva, Irina ; Möglich, Andreas ; Westenhoff, Sebastian:
Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor.
In: Structure. Vol. 25 (2017) . - pp. 1-6.
ISSN 0969-2126
DOI: https://doi.org/10.1016/j.str.2017.04.006

Abstract in another language

Light-oxygen-voltage (LOV) receptors are sensory proteins controlling a wide range of organismal adaptations in multiple kingdoms of life. Because of their modular nature, LOV domains are also attractive for use as optogenetic actuators. A flavin chromophore absorbs blue light, forms a bond with a proximal cysteine residue, and induces changes in the surroundings. There is a gap of knowledge on how this initial signal is relayed further through the sensor to the effector module. To characterize these conformational changes, we apply time-resolved X-ray scattering to the homodimeric LOV domain from Bacillus subtilis YtvA. We observe a global structural change in the LOV dimer synchronous with the formation of the chromophore photoproduct state. Using molecular modeling, this change is identified as splaying apart and relative rotation of the two monomers, which leads to an increased separation at the anchoring site of the effector modules.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: light-oxygen-voltage; Photocycle; protein structural dynamics; sensor histidine kinase; sensory photoreceptor; Signal Transduction; X-ray solution scattering
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 12 May 2017 06:04
Last Modified: 12 May 2017 06:04
URI: https://eref.uni-bayreuth.de/id/eprint/37116