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Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor

Titelangaben

Berntsson, Oskar ; Diensthuber, Ralph P. ; Panman, Matthijs R. ; Björling, Alexander ; Hughes, Ashley J. ; Henry, Léocadie ; Niebling, Stephan ; Newby, Gemma ; Liebi, Marianne ; Menzel, Andreas ; Henning, Robert ; Kosheleva, Irina ; Möglich, Andreas ; Westenhoff, Sebastian:
Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor.
In: Structure. Bd. 25 (2017) . - S. 1-6.
ISSN 0969-2126
DOI: 10.1016/j.str.2017.04.006

Abstract

Light-oxygen-voltage (LOV) receptors are sensory proteins controlling a wide range of organismal adaptations in multiple kingdoms of life. Because of their modular nature, LOV domains are also attractive for use as optogenetic actuators. A flavin chromophore absorbs blue light, forms a bond with a proximal cysteine residue, and induces changes in the surroundings. There is a gap of knowledge on how this initial signal is relayed further through the sensor to the effector module. To characterize these conformational changes, we apply time-resolved X-ray scattering to the homodimeric LOV domain from Bacillus subtilis YtvA. We observe a global structural change in the LOV dimer synchronous with the formation of the chromophore photoproduct state. Using molecular modeling, this change is identified as splaying apart and relative rotation of the two monomers, which leads to an increased separation at the anchoring site of the effector modules.

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Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: light-oxygen-voltage; Photocycle; protein structural dynamics; sensor histidine kinase; sensory photoreceptor; Signal Transduction; X-ray solution scattering
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie > Lehrstuhl Biochemie - Univ.-Prof. Dr. Andreas Möglich
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 12 Mai 2017 06:04
Letzte Änderung: 12 Mai 2017 06:04
URI: https://eref.uni-bayreuth.de/id/eprint/37116