Monodisperse collagen-gelatin beads as potential platforms for 3D cell culturing

Titelangaben

Ma, Shaohua ; Natoli, Manuela ; Liu, Xin ; Neubauer, Martin Peter ; Watt, Fiona M. ; Fery, Andreas ; Huck, Wilhelm T. S.:
Monodisperse collagen-gelatin beads as potential platforms for 3D cell culturing.
In: Journal of Materials Chemistry B. Bd. 1 (2013) Heft 38 . - S. 5128-5136.
ISSN 2050-7518
DOI: https://doi.org/10.1039/C3TB20851F

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Abstract

A droplet-based microfluidics technique is used to produce monodisperse, 80 mm collagen--gelatin beads with tunable mechanical properties in the range of 1--10 kPa after photo-crosslinking. The gel beads are porous, mechanically robust and stable in buffer, but can be degraded enzymatically. Encapsulated fibroblast cells maintain 70 viability after one-week encapsulation and preliminary results show that the degree of spreading of cells in gels is correlated with the stiffness of the material.