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Co-assemblies of micelle-forming diblock copolymers and enzymes on graphite substrate for an improved design of biosensor systems

Titelangaben

Sigolaeva, Larisa V. ; Pergushov, Dmitry V. ; Synatschke, Christopher V. ; Wolf, Andrea ; Dewald, Inna ; Kurochkin, Ilya N. ; Fery, Andreas ; Müller, Axel H. E.:
Co-assemblies of micelle-forming diblock copolymers and enzymes on graphite substrate for an improved design of biosensor systems.
In: Soft Matter. Bd. 9 (2013) Heft 10 . - S. 2858-2868.
ISSN 1744-6848
DOI: https://doi.org/10.1039/c2sm27298a

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Link zum Volltext (externe URL): Volltext

Abstract

The adsorption of ionic amphiphilic diblock copolymers comprising a polycationic block, polybutadiene- block-poly(2-(dimethylamino)ethyl methacrylate) (PB-b-PDMAEMA); and its quaternized derivative (PB-b- PDMAEMAq) from aqueous media onto graphite-based surfaces was examined. Both diblock copolymers in aqueous solution form star-like micelles with a hydrophobic PB core and a cationic corona built up from either strong cationic PDMAEMAq or pH-sensitive PDMAEMA. AFM experiments show that PB-b-PDMAEMAq micelles interact slightly with a graphite surface providing films with a low surface coverage. PB-b-PDMAEMA micelles adsorbed onto a graphite surface at pH 7 result in a more homogeneous coverage of the graphite surface by the diblock copolymer. The adsorption of two enzymes, tyrosinase (Tyr) and choline oxidase (ChO) on the graphite surface premodified with these diblock copolymers was also monitored by AFM and by electrochemical measurements of the enzymatic activities of PB-b-PDMAEMA--Tyr and PB-b-PDMAEMA--ChO films. A pronounced increase in the enzymatic activity of tyrosinase was observed with the increasing concentration of PB-b-PDMAEMA micelles in solution used for their depositions. Also, a pronounced increase in the enzymatic activities of both tyrosinase and choline oxidase was observed with the increasing pH of the deposition of the micelles from 2 to 10. The enzymatic activity increases with the coverage of the graphite surface with the preadsorbed copolymer. Finally, the polymer--enzyme films were tested as biosensors for phenol (when tyrosinase was adsorbed) and choline (when choline oxidase was adsorbed) and their activity and stability were compared to already existing setups.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren > Lehrstuhl Physikalische Chemie II - Univ.-Prof. Dr. Andreas Fery
Forschungseinrichtungen > Sonderforschungsbereiche, Forschergruppen > SFB 840 Von partikulären Nanosystemen zur Mesotechnologie
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Physikalische Chemie II
Forschungseinrichtungen
Forschungseinrichtungen > Sonderforschungsbereiche, Forschergruppen
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 540 Chemie
Eingestellt am: 05 Dec 2014 08:07
Letzte Änderung: 11 Feb 2015 08:34
URI: https://eref.uni-bayreuth.de/id/eprint/3944