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A detailed investigation of the formation kinetics and layer structure of poly(ethylene glycol) tether supported lipid bilayers

Titelangaben

Kaufmann, Stefan ; Papastavrou, Georg ; Kumar, Karthik ; Textor, Marcus ; Reimhult, Erik:
A detailed investigation of the formation kinetics and layer structure of poly(ethylene glycol) tether supported lipid bilayers.
In: Soft Matter. Bd. 5 (2009) Heft 14 . - S. 2804-2814.
ISSN 1744-6848
DOI: https://doi.org/10.1039/b901874c

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Link zum Volltext (externe URL): Volltext

Abstract

Supported lipid bilayers formed from liposomes containing phospholipids with covalently attached poly(ethylene glycol) (PEG-SLBs) potentially circumvent two current limitations of membrane sensor platforms, namely air-stability and limited aqueous space between the sensor substrate and the membrane. However, questions regarding the formation kinetics through self assembly and the presence of a PEG cushion underneath the lipid bilayer are still unanswered. Quartz crystal microbalance with dissipation monitoring and fluorescence recovery after photobleaching (FRAP) measurements show that PEG-SLB formation through self-assembly is possible in the mushroom regime while it is hindered in the brush regime. The observed dependence of the kinetics on grafting density and molecular weight of the PEG-lipids is attributed to the electrostatic and steric shielding effect of the enveloping PEG layer. In the mushroom phase, non-densely packed PEG does not completely screen lipid-surface interactions while in the brush phase, densely packed non-interacting PEG chains stabilise liposomes and prevent overall attractive interactions. Force-distance measurements are used to directly measure the thickness of the PEG-SLB structure and demonstrate the presence of PEG chains on both sides of the lipid bilayer. FRAP measurements support this finding by showing increased lipid mobility for increased PEG layer thickness through decoupling of the membrane from the substrate. Force-distance and FRAP measurements further reveal the mobility of the PEG-lipids and the novel detailed behavior of laterally mobile PEG mushrooms under mechanical compression.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Physikalische Chemie II
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren > Lehrstuhl Physikalische Chemie II - Univ.-Prof. Dr. Andreas Fery
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Physikalische Chemie II
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
Eingestellt am: 12 Sep 2017 11:06
Letzte Änderung: 12 Sep 2017 11:06
URI: https://eref.uni-bayreuth.de/id/eprint/39570