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Creating a Biomimetic Microenvironment for the Ex Vivo Expansion of Primary Human T Lymphocytes

Title data

Jérôme, Valérie ; Werner, Melanie ; Kaiser, Patrick ; Freitag, Ruth:
Creating a Biomimetic Microenvironment for the Ex Vivo Expansion of Primary Human T Lymphocytes.
In: Macromolecular Bioscience. (2017) .
ISSN 1616-5187
DOI: https://doi.org/10.1002/mabi.201700091

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

The ex vivo expansion of primary human T cells is of considerable interest. Current protocols call for the addition of massive amounts of stimuli. This study presents as alternative the expansion of such cells in semipermeable sodium cellulose sulfate/poly(diallyldimethyl) ammonium chloride (SCS/PDADMAC) polyelectrolyte microcapsules, which supports at least six cell divisions and results in >40 x 106 cells mLcapsule-1 within less than 10 d. Inside the microcapsules, the T cells are suspended in a viscous SCS-solution. The low molecular weight cut off (<10 000 Da) of the surrounding polyelectrolyte membrane assures that typical signaling molecules produced by the cells are retained, while nutrients and metabolites can pass. Expensive additives, such as interleukin-2 (IL-2), can be coencapsulated. Expansion then no longer requires specialized T-cell media. Moreover, these results suggest that an SCS with a low degree of sulfation has biomimetic properties, representing an artificial extracellular matrix mimicking heparin sulfate.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: biomimetic microenvironment; encapsulation; human T lymphocytes; microcultivation; polyelectrolyte
Subject classification: Biomimetic Microenvironment
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 600 Technology
600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 13 Sep 2017 10:52
Last Modified: 13 Sep 2017 10:52
URI: https://eref.uni-bayreuth.de/id/eprint/39622