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Biomimetic Systems shed Light on Actin-Based Motility Down to the Molecular Scale

Titelangaben

Romet-Lemonne, Guillaume ; Helfer, Emmanuele ; Delatour, Vincent ; Bugyi, Beata ; Bosch, Montserrat ; Romero, Stephane ; Carlier, Marie-France ; Schmidt, Stephan ; Fery, Andreas:
Biomimetic Systems shed Light on Actin-Based Motility Down to the Molecular Scale.
In: Biophysical Reviews and Letters. Bd. 4 (April 2009) Heft 1/2 . - S. 5-15.
ISSN 1793-7035
DOI: https://doi.org/10.1142/S1793048009000909

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Abstract

Cell motility, one of the modular activities of living cells, elicits the response of the cell to extra-cellular signals, to move directionally, feed, divide or transport materials. The combined actions of molecular motors and re-modeling of the cytoskeleton generate forces and movement. Here we describe mechanistic approaches of force and movement produced by site-directed assembly of actin filaments. The insight derived from a biochemical analysis of the protein machineries involved in "actin-based motile processes" like cell protrusions, invaginations, organelle propulsion, is used to build reconstituted assays that mimic cellular processes, using several protein machineries known to initiate filament assembly by different mechanisms. Reconstitution of complex self-organized systems presents a broad variety of interests. Reconstituting actin-based movement of a functionalized particle from a minimum number of pure proteins, first used to prove the general thermodynamic principles at work in motility, then was the basis for fully controlled physical measurements of forces produced by polymerization of actin against an obstacle and of the mechanical properties of the resulting polymer arrays. In addition, measurements at the mesoscopic scale (trajectories, velocity, polymer mechanics, fluorescence of specifically labeled components of the actin array, use of mutated proteins) can provide further insight into the molecular mechanisms underlying motility.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren > Lehrstuhl Physikalische Chemie II - Univ.-Prof. Dr. Andreas Fery
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Physikalische Chemie II
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige Professoren
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 540 Chemie
Eingestellt am: 08 Dec 2014 09:22
Letzte Änderung: 08 Dec 2014 09:22
URI: https://eref.uni-bayreuth.de/id/eprint/4001