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Associative nitrogen fixation in nodules of the conifer Lepidothamnus fonkii (Podocarpaceae) inhabiting ombrotrophic bogs in southern Patagonia

Title data

Borken, Werner ; Horn, Marcus A. ; Geimer, Stefan ; Bahamonde Aguilar, Nelson A. ; Knorr, Klaus-Holger:
Associative nitrogen fixation in nodules of the conifer Lepidothamnus fonkii (Podocarpaceae) inhabiting ombrotrophic bogs in southern Patagonia.
In: Scientific Reports. Vol. 6 (December 2016) . - No. 39072.
ISSN 2045-2322
DOI: https://doi.org/10.1038/srep39072

Abstract in another language

Biological N2 fixation (BNF) in the rhizosphere of Podocarpaceae is currently attributed to unspecific diazotrophs with negligible impact on N acquisition. Here, we report specific and high associative BNF in dead cells of root nodules of Lepidothamnus fonkii distributed in ombrotrophic peatlands of Patagonia. BNF of nodulated roots, intact plants of L. fonkii and rhizospheric peat was assessed by 15N2 and acetylene reduction. Diazotrophs were identified by electron microscopy, analysis of nitrogenase encoding genes (nifH) and transcripts, and 16S rRNA. Nitrogenase encoding nifH transcripts from root nodules point to Beijerinckiaceae (Rhizobiales), known as free-living diazotrophs. Electron microscopy and 16S rRNA analysis likewise identified active Beijerinckiaceae in outer dead cells of root nodules. NifH transcripts from the rhizopshere peat revealed diverse active diazotrophs including Beijerinckiaceae. Both methods revealed high activity of nitrogenase rates in cut roots of L. fonkii (2.5 μmol N g−1 d.w. d−1 based on 15N2 assay; 2.4 μmol C2H4 g−1 d.w. d−1 based on acetylene reduction assay). The data suggest that (i) nodules recruit diazotrophic Beijerinckiaceae from peat, (ii) dead nodule cells provide an exclusive habitat for Beijerinckiaceae, and (iii) BNF in L. fonkii is one potent pathway to overcome N deficiency in ombrotrophic peatlands of Patagonia.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER126145
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Professorship Cell Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Chair Soil Ecology
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 15 Jan 2018 10:31
Last Modified: 15 Jan 2018 10:31
URI: https://eref.uni-bayreuth.de/id/eprint/41326