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Küchler, Stefan ; Matsuura, Yu ; Dettner, Konrad ; Kikuchi, Yoshitomo:
Phylogenetically Diverse Burkholderia Associated with Midgut Crypts of Spurge Bugs, Dicranocephalus spp. (Heteroptera: Stenocephalidae).
In: Microbes and Environments.
Bd. 31
(2016)
Heft 2
.
- S. 145-153.
ISSN 1347-4405
DOI: https://doi.org/10.1264/jsme2.ME16042
Abstract
Diverse phytophagous heteropteran insects, commonly known as stinkbugs, are associated with specific gut symbioticbacteria, which have been found in midgut cryptic spaces. Recent studies have revealed that members of the stinkbug familiesCoreidae and Alydidae of the superfamily Coreoidea are consistently associated with a specific group of the betaproteobacterialgenus Burkholderia, called the “stinkbug-associated beneficial and environmental (SBE)” group, and horizontally acquirespecific symbionts from the environment every generation. However, the symbiotic system of another coreoid family,Stenocephalidae remains undetermined. We herein investigated four species of the stenocephalid genus Dicranocephalus.Examinations via fluorescence in situ hybridization (FISH) and transmission electron microscopy (TEM) revealed the typicalarrangement and ultrastructures of midgut crypts and gut symbionts. Cloning and molecular phylogenetic analyses of bacterialgenes showed that the midgut crypts of all species are colonized by Burkholderia strains, which were further assigned to differentsubgroups of the genus Burkholderia. In addition to the SBE-group Burkholderia, a number of stenocephalid symbiontsbelonged to a novel clade containing B. sordidicola and B. udeis, suggesting a specific symbiont clade for the Stenocephalidae.The symbiotic systems of stenocephalid bugs may provide a unique opportunity to study the ongoing evolution of symbiontassociations in the stinkbug-Burkholderia interaction.