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Phytochelatin Synthase has Contrasting Effects on Cadmium and Arsenic Accumulation in Rice Grains

Title data

Uraguchi, Shimpei ; Tanaka, Nobuhiro ; Hofmann, Christian ; Abiko, Kaho ; Ohkama-Ohtsu, Naoko ; Weber, Michael ; Kamiya, Takehiro ; Sone, Yuka ; Nakamura, Ryosuke ; Takanezawa, Yasukazu ; Kiyono, Masako ; Fujiwara, Toru ; Clemens, Stephan:
Phytochelatin Synthase has Contrasting Effects on Cadmium and Arsenic Accumulation in Rice Grains.
In: Plant & Cell Physiology. Vol. 58 (1 October 2017) Issue 10 . - pp. 1730-1742.
ISSN 1471-9053
DOI: https://doi.org/10.1093/pcp/pcx114

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Phytochelatin (PC) synthesis has been well demonstrated as a major metal tolerance mechanism in Arabidopsis thaliana, whereas its contribution to long-distance element transport especially in monocots remains elusive. Using rice as a cereal model, we examined physiological roles of Oryza sativa phytochelatin synthase 1 (OsPCS1) in the distribution and detoxification of arsenic (As) and cadmium (Cd), two toxic elements associated with major food safety concerns. First, we isolated four different transcript variants of OsPCS1 as well as one from OsPCS2. Quantitative real-time reverse transcription-PCR (RT-PCR) of each OsPCS transcript in rice seedlings suggested that expression of OsPCS1full, the longest OsPCS1 variant, was most abundant, followed by OsPCS2. Heterologous expression of OsPCS variants in PCS-deficient mutants of Schizosaccharomyces pombe and A. thaliana suggested that OsPCS1full possessed PCS activity in response to As(III) and Cd while the activity of other PCS variants was very low. To address physiological functions in toxic element tolerance and accumulation, two independent OsPCS1 mutant rice lines (a T-DNA and a Tos17 insertion line) were identified. The OsPCS1 mutants exhibited increased sensitivity to As(III) and Cd in hydroponic experiments, showing the importance of OsPCS1-dependent PC synthesis for rice As(III) and Cd tolerance. Elemental analyses of rice plants grown in soil with environmentally relevant As and Cd concentrations showed increased As accumulation and decreased Cd accumulation in grains of the T-DNA line. The Tos17 mutant also exhibited the reduced Cd accumulation phenotype. These contrasting effects on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER142181
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 570 Life sciences, biology
500 Science > 580 Plants (Botany)
Date Deposited: 09 Jan 2018 14:53
Last Modified: 11 Jan 2018 10:52
URI: https://eref.uni-bayreuth.de/id/eprint/41552