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Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D

Title data

Graf, Christopher G. F. ; Schulz, Christian ; Schmalzlein, Marina ; Heinlein, Christian ; Mönnich, Manuel ; Perkams, Lukas ; Püttner, Markus ; Boos, Irene ; Hessefort, Markus ; Lombana Sanchez, Jose Nelson ; Weyand, Michael ; Steegborn, Clemens ; Breiden, Bernadette ; Ross, Kerstin ; Schwarzmann, Günter ; Sandhoff, Konrad ; Unverzagt, Carlo:
Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D.
In: Angewandte Chemie International Edition. Vol. 56 (2 June 2017) Issue 19 . - pp. 5252-5257.
ISSN 1521-3773
DOI: https://doi.org/10.1002/anie.201701362

Abstract in another language

The main glycoforms of the hydrophobic lysosomal glycoprotein saposin D (SapD) were synthesized by native chemical ligation. An approach for the challenging solid-phase synthesis of the fragments was developed. Three SapD glycoforms were obtained following a general and robust refolding and purification protocol. A crystal structure of one glycoform confirmed its native structure and disulfide pattern. Functional assays revealed that the lipid-binding properties of three SapD glycoforms are highly affected by the single sugar moiety of SapD showing a dependency of the size and the type of N-glycan.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 13 Feb 2018 08:22
Last Modified: 13 Feb 2018 08:22
URI: https://eref.uni-bayreuth.de/id/eprint/42262