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Self-Assembly of Spider Silk-Fusion Proteins Comprising Enzymatic and Fluorescence Activity

Titelangaben

Humenik, Martin ; Mohrand, Madeleine ; Scheibel, Thomas:
Self-Assembly of Spider Silk-Fusion Proteins Comprising Enzymatic and Fluorescence Activity.
In: Bioconjugate Chemistry. (2018) . - 7 S..
ISSN 1520-4812
DOI: https://doi.org/10.1021/acs.bioconjchem.7b00759

Abstract

The recombinant spider silk protein eADF4(C16) was genetically fused either with esterase 2 (EST2) or green fluorescent protein (GFP). The fusions EST-eADF4(C16) and GFP-eADF4(C16) were spectroscopically investigated and showed native structures of EST and GFP. The structural integrity was confirmed by the enzymatic activity of EST and the fluorescence of GFP. The spider silk moiety retained its intrinsically unstructured conformation in solution and the self-assembly into either nanofibrils or nanoparticles could be controlled by the concentration of phosphate. Particles, however, showed significantly lower activity of the EST and GFP domains likely caused by a steric hindrance. However, upon self-assembly of EST-eADF4-(C16) and GFP-eADF4(C16) into fibrils the protein activities were retained. In general, the fusion of globular enzymes with the spider silk domain allows the generation of fibrous biomaterials with catalytic or light emitting properties.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Spider Silk Protein; Fusion; EST; GFP; steric hindrance; catalytic
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien > Lehrstuhl Biomaterialien - Univ.-Prof. Dr. Thomas Scheibel
Profilfelder
Profilfelder > Advanced Fields
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Profilfelder > Advanced Fields > Neue Materialien
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Profilfelder > Emerging Fields
Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften
Forschungseinrichtungen
Forschungseinrichtungen > Forschungszentren
Forschungseinrichtungen > Forschungszentren > Bayreuther Materialzentrum - BayMAT
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 15 Feb 2018 08:00
Letzte Änderung: 15 Feb 2018 08:00
URI: https://eref.uni-bayreuth.de/id/eprint/42278