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Optogenetic Control by Pulsed Illumination

Title data

Hennemann, Julia ; Iwasaki, Roman S. ; Grund, Tamara N. ; Diensthuber, Ralph P. ; Richter, Florian ; Möglich, Andreas:
Optogenetic Control by Pulsed Illumination.
In: ChemBioChem. (2018) .
ISSN 1439-7633
DOI: https://doi.org/10.1002/cbic.201800030

Official URL: Volltext

Abstract in another language

Sensory photoreceptors evoke numerous adaptive responses in Nature and serve as light-gated actuators in optogenetics to enable the spatiotemporally precise, reversible and noninvasive control of cellular events. The output of optogenetic circuits can often be dialed in by varying illumination quality, quantity and duration. Here, we devise a programmable matrix of light-emitting diodes to efficiently probe the response of optogenetic systems to intermittently applied light of varying intensity and pulse frequency. Circuits for light-regulated gene expression markedly differed in their responses to pulsed illumination of a single color which sufficed for sequentially triggering them. In addition to quantity and quality, the pulse frequency of intermittent light hence provides a further input variable for output control in optogenetics and photobiology. Pulsed illumination schemes allow the reduction of overall light dose and facilitate the multiplexing of several light-dependent actuators and reporters.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: synthetic biology; light-oxygen-voltage; optogenetics; sensory photoreceptor; signal transduction
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 15 Feb 2018 09:58
Last Modified: 15 Feb 2018 09:58
URI: https://eref.uni-bayreuth.de/id/eprint/42296