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Epifluorescence, confocal and total internal reflection microscopy for single-molecule experiments : a quantitative comparison

Title data

Lang, Erwin ; Baier, Jürgen ; Köhler, Jürgen:
Epifluorescence, confocal and total internal reflection microscopy for single-molecule experiments : a quantitative comparison.
In: Journal of Microscopy. Vol. 222 (2006) Issue 2 . - pp. 118-123.
ISSN 1365-2818
DOI: https://doi.org/10.1111/j.1365-2818.2006.01579.x

Abstract in another language

Epifluorescence, confocal and total internal reflection microscopy are the most widely used techniques for optical single-molecule experiments. Employing these methods, we recorded the emission intensity of the same single molecule as a function of the excitation rate under otherwise identical experimental conditions. Evaluation of these data provides a quantitative comparison of the signal-to-background ratios that can be achieved for the three microscopic techniques.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: TIR microscopy
Institutions of the University: Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie - Univ.-Prof. Dr. Jürgen Köhler
Faculties
Faculties > Faculty of Mathematics, Physics und Computer Science
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics
Faculties > Faculty of Mathematics, Physics und Computer Science > Department of Physics > Lehrstuhl Experimentalphysik IX - Spektroskopie weicher Materie
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 530 Physics
Date Deposited: 12 Apr 2018 11:45
Last Modified: 12 Apr 2018 11:45
URI: https://eref.uni-bayreuth.de/id/eprint/43361