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Engineered hybrid spider silk particles as delivery system for peptide vaccines

Title data

Lucke, Matthias ; Mottas, Inès ; Herbst, Tina ; Hotz, Christian ; Römer, Lin ; Schierling, Martina ; Herold, Heike M. ; Slotta, Ute ; Spinetti, Thibaut ; Scheibel, Thomas ; Winter, Gerhard ; Bourquin, Carol ; Engert, Julia:
Engineered hybrid spider silk particles as delivery system for peptide vaccines.
In: Biomaterials. Vol. 172 (21 April 2018) . - pp. 105-115.
ISSN 1878-5905
DOI: https://doi.org/10.1016/j.biomaterials.2018.04.008

Abstract in another language

The generation of strong T-cell immunity is one of the main challenges for the development of successfulvaccines against cancer and major infectious diseases. Here we have engineered spider silk particles as delivery system for a peptide-based vaccination that leads to effective priming of cytotoxic T-cells. The recombinant spider silk protein eADF4(C16) was fused to the antigenic peptide from ovalbumin, either without linker or with a cathepsin cleavable peptide linker. Particles prepared from the hybrid proteins were taken up by dendritic cells, which are essential for T-cell priming, and successfully activated cytotoxic T-cells, without signs of immunotoxicity or unspecific immunostimulatory activity. Upon subcutaneous injection in mice, the particles were taken up by dendritic cells and accumulated in the lymph nodes, where immune responses are generated. Particles from hybrid proteins containing a cathepsin-cleavable linker induced a strong antigen-specific proliferation of cytotoxic T-cells in vivo, even in the absence of a vaccine adjuvant. We thus demonstrate the efficacy of a new vaccine strategy using a protein-based all-in-one vaccination system, where spider silk particles serve as carriers with an incorporated peptide antigen. Our study further suggests that engineered spider silk-based vaccines are extremely stable, easy to manufacture, and readily customizable.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Vaccine delivery; Antigen delivery; Peptide vaccines; Cytotoxic T-cells; Recombinant silk protein
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 05 Jul 2018 13:46
Last Modified: 05 Jul 2018 13:46
URI: https://eref.uni-bayreuth.de/id/eprint/44978