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Engineered hybrid spider silk particles as delivery system for peptide vaccines

Titelangaben

Lucke, Matthias ; Mottas, Inès ; Herbst, Tina ; Hotz, Christian ; Römer, Lin ; Schierling, Martina ; Herold, Heike M. ; Slotta, Ute ; Spinetti, Thibaut ; Scheibel, Thomas ; Winter, Gerhard ; Bourquin, Carol ; Engert, Julia:
Engineered hybrid spider silk particles as delivery system for peptide vaccines.
In: Biomaterials. Bd. 172 (21 April 2018) . - S. 105-115.
ISSN 1878-5905
DOI: https://doi.org/10.1016/j.biomaterials.2018.04.008

Abstract

The generation of strong T-cell immunity is one of the main challenges for the development of successfulvaccines against cancer and major infectious diseases. Here we have engineered spider silk particles as delivery system for a peptide-based vaccination that leads to effective priming of cytotoxic T-cells. The recombinant spider silk protein eADF4(C16) was fused to the antigenic peptide from ovalbumin, either without linker or with a cathepsin cleavable peptide linker. Particles prepared from the hybrid proteins were taken up by dendritic cells, which are essential for T-cell priming, and successfully activated cytotoxic T-cells, without signs of immunotoxicity or unspecific immunostimulatory activity. Upon subcutaneous injection in mice, the particles were taken up by dendritic cells and accumulated in the lymph nodes, where immune responses are generated. Particles from hybrid proteins containing a cathepsin-cleavable linker induced a strong antigen-specific proliferation of cytotoxic T-cells in vivo, even in the absence of a vaccine adjuvant. We thus demonstrate the efficacy of a new vaccine strategy using a protein-based all-in-one vaccination system, where spider silk particles serve as carriers with an incorporated peptide antigen. Our study further suggests that engineered spider silk-based vaccines are extremely stable, easy to manufacture, and readily customizable.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Vaccine delivery; Antigen delivery; Peptide vaccines; Cytotoxic T-cells; Recombinant silk protein
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien > Lehrstuhl Biomaterialien - Univ.-Prof. Dr. Thomas Scheibel
Profilfelder
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Profilfelder > Advanced Fields > Neue Materialien
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Forschungseinrichtungen > Forschungszentren > Bayreuther Materialzentrum - BayMAT
Profilfelder > Advanced Fields
Forschungseinrichtungen
Forschungseinrichtungen > Forschungszentren
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 05 Jul 2018 13:46
Letzte Änderung: 05 Jul 2018 13:46
URI: https://eref.uni-bayreuth.de/id/eprint/44978