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Scale‐up of the ex vivo expansion of encapsulated primary human T lymphocytes

Titelangaben

Kaiser, Patrick ; Werner, Melanie ; Jérôme, Valérie ; Freitag, Ruth:
Scale‐up of the ex vivo expansion of encapsulated primary human T lymphocytes.
In: Biotechnology and Bioengineering. (30 Juni 2018) Heft Early view . - S. 1-11.
ISSN 1097-0290
DOI: https://doi.org/10.1002/bit.26786

Abstract

A number of evolving medical therapies call for the controlled expansion of primary human T lymphocytes. After encapsulation in sodium cellulose sulfate–poly(diallyldimethyl) ammonium chloride polyelectrolyte capsules, T lymphocytes can be expanded without persisting activation. Here, the challenge of scaling up this process is addressed. Encapsulated T lymphocytes were cultured in spinner flasks as well as in several types of the bioreactor, including fixed and fluidized beds, a waved cell bag, and a standard stirred tank reactor (STR; 1‐L scale). Two proprietary T lymphocyte culture media as well as a standard RPMI‐based medium were used. As before, encapsulation coincided with the presence of only a low fraction of activated T lymphocytes (peripheral blood T cells) in the total population. Unexpectedly, growth rates were lower in well‐mixed reactors than those in cultivations under static conditions, that is, in T‐flasks. Switching the STR to low oxygen conditions (40% air saturation) improved the growth rates to the level of the static cultures and thus forms the potential basis for efficient scale‐up of T lymphocyte expansion. Results are presented on the ex vivo expansion of primary human T cells in T‐flasks and spinner flasks, as well as in several types of the bioreactor, including packed and fluidized beds, waved cell bags, and a standard stirred tank reactor.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Zusätzliche Informationen: Online version of record before inclusion in an issue
Keywords: biomimetic microenvironment; bioreactor; encapsulation; human T lymphocytes; polyelectrolyte
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 600 Technik
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
Eingestellt am: 25 Jul 2018 08:30
Letzte Änderung: 25 Jul 2018 08:30
URI: https://eref.uni-bayreuth.de/id/eprint/45236