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Scale‐up of the ex vivo expansion of encapsulated primary human T lymphocytes

Title data

Kaiser, Patrick ; Werner, Melanie ; Jérôme, Valérie ; Freitag, Ruth:
Scale‐up of the ex vivo expansion of encapsulated primary human T lymphocytes.
In: Biotechnology and Bioengineering. (30 June 2018) Issue Early view . - pp. 1-11.
ISSN 1097-0290
DOI: https://doi.org/10.1002/bit.26786

Abstract in another language

A number of evolving medical therapies call for the controlled expansion of primary human T lymphocytes. After encapsulation in sodium cellulose sulfate–poly(diallyldimethyl) ammonium chloride polyelectrolyte capsules, T lymphocytes can be expanded without persisting activation. Here, the challenge of scaling up this process is addressed. Encapsulated T lymphocytes were cultured in spinner flasks as well as in several types of the bioreactor, including fixed and fluidized beds, a waved cell bag, and a standard stirred tank reactor (STR; 1‐L scale). Two proprietary T lymphocyte culture media as well as a standard RPMI‐based medium were used. As before, encapsulation coincided with the presence of only a low fraction of activated T lymphocytes (peripheral blood T cells) in the total population. Unexpectedly, growth rates were lower in well‐mixed reactors than those in cultivations under static conditions, that is, in T‐flasks. Switching the STR to low oxygen conditions (40% air saturation) improved the growth rates to the level of the static cultures and thus forms the potential basis for efficient scale‐up of T lymphocyte expansion. Results are presented on the ex vivo expansion of primary human T cells in T‐flasks and spinner flasks, as well as in several types of the bioreactor, including packed and fluidized beds, waved cell bags, and a standard stirred tank reactor.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: Online version of record before inclusion in an issue
Keywords: biomimetic microenvironment; bioreactor; encapsulation; human T lymphocytes; polyelectrolyte
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 600 Technology
600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 25 Jul 2018 08:30
Last Modified: 25 Jul 2018 08:30
URI: https://eref.uni-bayreuth.de/id/eprint/45236