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The universally-conserved transcription factor RfaH is recruited to a hairpin structure of the non-template DNA strand

Title data

Zuber, Philipp ; Artsimovitch, Irina ; NandyMazumdar, Monali ; Liu, Zhaokun ; Nedialkov, Yuri ; Schweimer, Kristian ; Rösch, Paul ; Knauer, Stefan:
The universally-conserved transcription factor RfaH is recruited to a hairpin structure of the non-template DNA strand.
In: eLIFE. Vol. 7 (May 2018) .
ISSN 2050-084X
DOI: https://doi.org/10.7554/eLife.36349

Abstract in another language

RfaH, a transcription regulator of the universally conserved NusG/Spt5 family, utilizes a unique mode of recruitment to elongating RNA polymerase to activate virulence genes. RfaH function depends critically on an ops sequence, an exemplar of a consensus pause, in the non-template DNA strand of the transcription bubble. We used structural and functional analyses to elucidate the role of ops in RfaH recruitment. Our results demonstrate that ops induces pausing to facilitate RfaH binding and establishes direct contacts with RfaH. Strikingly, the non-template DNA forms a hairpin in the RfaH:ops complex structure, flipping out a conserved T residue that is specifically recognized by RfaH. Molecular modeling and genetic evidence support the notion that ops hairpin is required for RfaH recruitment. We argue that both the sequence and the structure of the non-template strand are read out by transcription factors, expanding the repertoire of transcriptional regulators in all domains of life.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 540 Chemistry
Date Deposited: 21 Dec 2018 08:01
Last Modified: 30 Jan 2019 09:35
URI: https://eref.uni-bayreuth.de/id/eprint/46333