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Solution structure of the DNA-binding domain of the yeast transcriptional activator protein GCN4

Title data

Saudek, Vladimir ; Pastore, Annalisa ; Castiglione Morelli, Maria A. ; Frank, Rainer ; Gausepohl, Heinrich ; Gibson, Toby ; Weih, Falk ; Rösch, Paul:
Solution structure of the DNA-binding domain of the yeast transcriptional activator protein GCN4.
In: Protein Engineering Design & Selection. Vol. 4 (1990) Issue 1 . - pp. 3-10.
ISSN 0269-2139
DOI: https://doi.org/10.1093/protein/4.1.3

Abstract in another language

The solution structure of an active synthetic peptide containing both the leucine zipper and the adjacent basic domain of the yeast transcription factor GCN4 (residues 220–280) was determined by NMR. The two domains show structurally distinct behaviours. In the absence of DNA, the basic domain is, although very flexible, structured and fluctuating around a helical conformation. The leucine zipper region forms a long, uninterrupted helix. From a suitable set of NMR distances the three-dimensional structure of the leucine zipper monomeric sub-domain was calculated by distance geometry algorithms. The structure of the symmetrical parallel dimer was obtained by model building using the NMR information. A smaller peptide with the sequence of the isolated basic region (residues 1–35 of the 61 residue peptide) was also synthesized. Circular dichroism studies showed 30–40% helicity. A flexible helix spans the region between residues 8 and 21. The comparison of our results with suggested models is discussed in detail.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 21 Dec 2018 08:17
Last Modified: 16 May 2019 05:37
URI: https://eref.uni-bayreuth.de/id/eprint/46765