Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

AZT-resistant foamy virus

Title data

Kretzschmar, Benedikt ; Nowrouzi, Ali ; Hartl, Maximilian J. ; Gärtner, Kathleen ; Wiktorowicz, Tatiana ; Herchenröder, Ottmar ; Kanzler, Sylvia ; Rudolph, Wolfram ; Mergia, Ayalew ; Wöhrl, Birgitta M. ; Rethwilm, Axel:
AZT-resistant foamy virus.
In: Virology. Vol. 370 (January 2008) Issue 1 . - pp. 151-157.
ISSN 0042-6822
DOI: https://doi.org/10.1016/j.virol.2007.08.025

Abstract in another language

Azidothymidine (AZT) is a reverse transcriptase (RT) inhibitor that efficiently blocks the replication of spumaretroviruses or foamy viruses (FVs). To more precisely elucidate the mechanism of action of the FV RT enzyme, we generated an AZT-resistant FV in cell culture. Biologically resistant virus was obtained for simian foamy virus from macaque (SFVmac), which was insensitive to AZT concentrations of 1 mM, but not for FVs derived from chimpanzees. Nucleotide sequencing revealed four non-silent mutations in the pol gene. Introduction of these mutations into an infectious molecular clone identified all changes to be required for the fully AZT-resistant phenotype of SFVmac. The alteration of individual sites showed that AZT resistance in SFVmac was likely acquired by consecutive acquisition of pol mutations in a defined order, because some alterations on their own did not result in an efficiently replicating virus, neither in the presence nor in the absence of AZT. The introduction of the mutations into the RT of the closely related prototypic FV (PFV) did not yield an AZT-resistant virus, instead they significantly impaired the viral fitness.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 29 Jan 2019 11:36
Last Modified: 15 May 2019 08:32
URI: https://eref.uni-bayreuth.de/id/eprint/47110