Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

CAX1 suppresses Cd-induced generation of reactive oxygen species in Arabidopsis halleri

Title data

Ahmadi, Hassan ; Corso, Massimiliano ; Weber, Michael ; Verbruggen, Nathalie ; Clemens, Stephan:
CAX1 suppresses Cd-induced generation of reactive oxygen species in Arabidopsis halleri.
In: Plant, Cell & Environment. Vol. 41 (2018) Issue 10 . - pp. 2435-2448.
ISSN 1365-3040
DOI: https://doi.org/10.1111/pce.13362

Abstract in another language

The molecular analysis of metal hyperaccumulation in species such as Arabidopsis halleri offers the chance to gain insights into metal homeostasis and into the evolution of adaptation to extreme habitats. A prerequisite of metal hyperaccumulation is metal hypertolerance. Genetic analysis of a backcross population derived from A. lyrata x A. halleri crosses revealed three quantitative trait loci (QTLs) for Cd hypertolerance. A candidate gene for Cdtol2 is AhCAX1, encoding a vacuolar Ca2+/H+ antiporter. We developed a method for the transformation of vegetatively propagated A. halleri plants and generated AhCAX1-silenced lines. Upon Cd2+ exposure several-fold higher accumulation of reactive oxygen species (ROS) was detectable in roots of AhCAX1-silenced plants. In accordance with the dependence of Cdtol2 on external Ca2+ concentration, this phenotype was exclusively observed in low Ca2+ conditions. The effects of external Ca2+ on Cd accumulation cannot explain the phenotype as they were not influenced by the genotype. Our data strongly support the hypothesis that higher expression of CAX1 in A. halleri relative to other Arabidopsis species represents a Cd hypertolerance factor. We propose a function of AhCAX1 in preventing a positive feedback loop of Cd-elicited ROS production triggering further Ca2+-dependent ROS accumulation.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER146377
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Mar 2019 08:11
Last Modified: 29 Mar 2019 08:11
URI: https://eref.uni-bayreuth.de/id/eprint/48133