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Preparation of Bacterial Magnetosomes for Proteome Analysis

Title data

Raschdorf, Oliver ; Schüler, Dirk ; Uebe, René:
Preparation of Bacterial Magnetosomes for Proteome Analysis.
In: Becher, Dörte (ed.): Microbial Proteomics : Methods and Protocols. - New York, NY : Springer New York , 2018 . - pp. 45-57 . - (Methods in Molecular Biology ; 1841 )
ISBN 978-1-4939-8695-8
DOI: https://doi.org/10.1007/978-1-4939-8695-8_5

Official URL: Volltext

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Magnetotactic bacteria form unique prokaryotic organelles, termed magnetosomes, which consist of membrane-enclosed magnetite nanoparticles. Analysis of magnetosome biogenesis has been greatly facilitated by proteomic methods. These, however, require pure, highly enriched magnetosomes. Here, we describe the purification of magnetosomes from Magnetospirillum gryphiswaldense using high pressure cell disruption, and sequential purification by magnetic enrichment and sucrose density ultracentrifugation. The resulting enriched magnetosomes can be subsequently subjected to proteomic analyses or biotechnological applications.

Further data

Item Type: Article in a book
Refereed: Yes
Keywords: Prokaryotic organelles; Magnetosomes; Magnetite nanoparticles; Magnetic separation; Ultracentrifugation; Biotechnological application
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 05 Apr 2019 07:26
Last Modified: 09 Apr 2019 07:27
URI: https://eref.uni-bayreuth.de/id/eprint/48561