Preparation of Bacterial Magnetosomes for Proteome Analysis

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Raschdorf, Oliver ; Schüler, Dirk ; Uebe, René:
Preparation of Bacterial Magnetosomes for Proteome Analysis.
In: Becher, Dörte (Hrsg.): Microbial Proteomics : Methods and Protocols. - New York : Springer , 2018 . - S. 45-57 . - (Methods in Molecular Biology ; 1841 )
ISBN 978-1-4939-8695-8
DOI: https://doi.org/10.1007/978-1-4939-8695-8_5

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Abstract

Magnetotactic bacteria form unique prokaryotic organelles, termed magnetosomes, which consist of membrane-enclosed magnetite nanoparticles. Analysis of magnetosome biogenesis has been greatly facilitated by proteomic methods. These, however, require pure, highly enriched magnetosomes. Here, we describe the purification of magnetosomes from Magnetospirillum gryphiswaldense using high pressure cell disruption, and sequential purification by magnetic enrichment and sucrose density ultracentrifugation. The resulting enriched magnetosomes can be subsequently subjected to proteomic analyses or biotechnological applications.