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Generation of Multifunctional Magnetic Nanoparticles with Amplified Catalytic Activities by Genetic Expression of Enzyme Arrays on Bacterial Magnetosomes

Title data

Mickoleit, Frank ; Schüler, Dirk:
Generation of Multifunctional Magnetic Nanoparticles with Amplified Catalytic Activities by Genetic Expression of Enzyme Arrays on Bacterial Magnetosomes.
In: Advanced Biosystems. Vol. 2 (November 2017) Issue 1 . - No. 1700109.
ISSN 2366-7478
DOI: https://doi.org/10.1002/adbi.201700109

Official URL: Volltext

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Project information

Project title:
Project's official titleProject's id
DFG Schwerpunktprogramm SPP 1569 "Generation of multifunctional inorganic materials by molecular bionics"Schu 1080/15-3
ERC AdG Syntomagx692637

Project financing: Deutsche Forschungsgemeinschaft
European Research Council (ERC) Advanced Grants

Abstract in another language

Abstract Due to their highly regulated biosynthesis, magnetosomes biomineralized by magnetotactic bacteria represent natural magnetic nanoparticles with unique physical and chemical properties. They consist of a magnetite core that is surrounded by a biological membrane and are therefore reminiscent to magnetic “core–shell” nanoparticles. Their usability in many nanotechnological and biomedical applications would be further improved by the introduction of additional catalytic and imaging modalities. Here, a new in vivo strategy is explored for magnetosome display of foreign polypeptides with maximized protein-to-particle ratios. Arrays of up to five monomers of the model enzyme glucuronidase GusA plus the additional fluorophore mEGFP are genetically fused as single large hybrid proteins to highly expressed magnetosome protein anchors. In total, about 190 GusA monomers are covalently attached to individual particles. Assuming layers of GusA rows surrounding the particles, the monomers would thus cover up to 90% of the magnetosome surface. The approach generates nanoparticles that exhibit magnetism, fluorescence, and stable catalytic activities, which are stepwise increased with the number of GusA monomers. In summary, multicopy expression of arrayed foreign proteins represents a powerful methodology for the biosynthesis of tailored biohybrid magnetic nanoparticles with several genetically encoded and tunable functionalities.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: bacterial nanoparticles; enzyme kinetics; glucuronidase; magnetosome expression; surface functionalization
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Result of work at the UBT: Yes
DDC Subjects: 300 Social sciences > 320 Political science
300 Social sciences > 330 Economics
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 15 Apr 2019 07:04
Last Modified: 15 Apr 2019 07:08
URI: https://eref.uni-bayreuth.de/id/eprint/48684