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Nuclease footprinting of human immunodeficiency virus reverse transcriptase/tRNA(Lys-3) complexes

Title data

Wöhrl, Birgitta ; Ehresmann, Bernard ; Keith, Gerhard ; Le Grice, Stuart F.J.:
Nuclease footprinting of human immunodeficiency virus reverse transcriptase/tRNA(Lys-3) complexes.
In: The Journal of Biological Chemistry. Vol. 268 (1993) Issue 18 . - pp. 13617-13624.
ISSN 1083-351X

Official URL: Volltext

Abstract in another language

Nuclease footprinting has been used to probe features of binary complexes of type 1 human immunodeficiency virus reverse transcriptase (HIV-1 RT) with both natural and synthetic preparations of its cognate replication primer, tRNA(Lys-3). In addition to heterodimeric RT (p66/p51), ribonucleoprotein complexes containing either the p66 or p51 subunit were analyzed. Footprinting experiments employed both structure- and sequence-specific nucleases. Our results indicate a similar mode of interaction for the three RT preparations tested, suggesting contact with each loop of the tRNA primer (D, anticodon, and T psi C), as well as minor perturbation of the anticodon stem. Although there is little evidence for extensive disruption of the 3'-acceptor stem. RNase A footprinting data with natural and synthetic tRNA suggests that potential base pairing between the T psi C and D loops is disrupted in the presence of RT.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Faculties
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 20 May 2019 12:02
Last Modified: 20 May 2019 12:02
URI: https://eref.uni-bayreuth.de/id/eprint/49006