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Fluorimetric detection of protoporphyrins as an indicator for quality monitoring of fresh intact pork meat

Title data

Schneider, J. ; Wulf, J. ; Surowsky, B. ; Schmidt, Heinar ; Schwägele, Fredi ; Schlüter, Oliver:
Fluorimetric detection of protoporphyrins as an indicator for quality monitoring of fresh intact pork meat.
In: Meat Science. Vol. 80 (December 2008) Issue 4 . - pp. 1320-1325.
ISSN 1873-4138
DOI: https://doi.org/10.1016/j.meatsci.2008.06.007

Abstract in another language

In fresh meat production fast and non-destructive quality monitoring along the distribution chain is a key aspect to guaranteeing high quality and safe products for consumption. The applicability of fluorescence spectroscopy using protoporphyrins as indicators for meat ageing was investigated. Porcine musculus longissimus dorsi (MLD) was stored in slices over 20 days at 5 and 12 degrees C and measured every day with an excitation of 420 nm and an emission range of 550-750 nm. Additionally, pH, drip loss and colour were examined to assess possible correlations. The obtained spectra of the MLD showed an increase in three peaks at 592, 638 and 705 nm which could be reconstructed using the spectra of standard solutions of protoporphyrin IX (PP) and zinc protoporphyrin IX (ZnPP) or magnesium protoporphyrin (MgPP), respectively. Using principal component analysis (PCA) on the fluorescence spectral data, the meat slices stored at 5 degrees C showed differences in the fluorescence signal after the 10th day and 5th day when stored at 12 degrees C. An interrelationship between the additional analyses and the fluorescence intensities on these relevant days could not be established. In conclusion, the increase of ZnPP fluorescence due to temperature related changes of physiological meat properties is capable of serving as a quality indicator with regards to inadequate conditioning (e.g. during transportation and/or storage) of pork meat.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: ISI:000260294000051
Keywords: Fluorescence spectroscopy; Pork; Zinc protoporphyrin IX; Protoporphyrin IX; Non-invasive Quality monitoring
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Lehrstuhl Bioanalytik und Lebensmittelanalytik > Lehrstuhl Bioanalytik und Lebensmittelanalytik - Univ.-Prof. Dr. Andreas Römpp
Graduate Schools > Bayreuth Graduate School of Mathematical and Natural Sciences (BayNAT) > Molecular Biosciences
Result of work at the UBT: No
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 30 Jul 2019 11:26
Last Modified: 30 Jul 2019 11:26
URI: https://eref.uni-bayreuth.de/id/eprint/51627