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The therapeutically anti-prion active antibody-fragment scFv-W226 : paramagnetic relaxation-enhanced NMR spectroscopy aided structure elucidation of the paratope-epitope interface

Title data

Mangels, Christian ; Kellner, Ruth ; Einsiedel, Jürgen ; Weiglmeier, Philipp Richard ; Rösch, Paul ; Gmeiner, Peter ; Schwarzinger, Stephan:
The therapeutically anti-prion active antibody-fragment scFv-W226 : paramagnetic relaxation-enhanced NMR spectroscopy aided structure elucidation of the paratope-epitope interface.
In: Journal of Biomolecular Structure & Dynamics. Vol. 28 (2010) Issue 1 . - pp. 13-22.
ISSN 0739-1102

Abstract in another language

Antibodies have become indispensable reagents with numerous applications in biological and biotechnical analysis, in diagnostics as well as in therapy. In all cases, selective interaction with an epitope is crucial and depends on the conformation of the paratope. While epitopes are routinely mapped at high throughput, methods revealing structural insights on a rather short timescale are rare. We here demonstrate paramagnetic relaxation-enhanced (PRE) NMR spectroscopy to be a powerful tool unraveling structural information about epitope-orientation in a groove spanned by the complementary determining regions. In particular, we utilize the spin label TOAC, which is fused to the peptidic epitope using standard solid-phase chemistry and which is characterized by a reduced mobility compared to, e.g., spin labels attached to the side-chain functionalities of cysteine or lysine residues. We apply the method to determine the orientation of helix 1 of the prion protein, which is the epitope for the therapeutically anti-prion active scFv fragment W226.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 19 Dec 2014 14:19
Last Modified: 20 Jan 2015 10:18
URI: https://eref.uni-bayreuth.de/id/eprint/5325