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Recombinant Spider Silk and Collagen-Based Nerve GuidanceConduits Support Neuronal Cell Differentiation and Functionality inVitro

Title data

Pawar, Kiran ; Welzel, Georg ; Haynl, Christian ; Schuster, Stefan ; Scheibel, Thomas:
Recombinant Spider Silk and Collagen-Based Nerve GuidanceConduits Support Neuronal Cell Differentiation and Functionality inVitro.
In: Applied Bio Materials. Vol. 2 (3 October 2019) Issue 11 . - pp. 4872-4880.
ISSN 2576-6422
DOI: https://doi.org/10.1021/acsabm.9b00628

Abstract in another language

Biomaterial scaffolds are under investigation as therapeutic tools to bridge nerve endings following traumatic peripheral nerve injury. The goal is to develop biocompatible nerve guidance conduits (NGCs) with internal guiding structures that promote longitudinally oriented cell migration and regeneration. In the present study, a nonwoven mesh (NWM) made of a recombinant spider silk protein was processed into a tubular structure, ensuring structural integrity of enclosed microfluidics-produced collagen fibers for cell and neurite guidance. The differentiated type of the neuroblastoma X glioma hybrid cell line NG108-15 was used as a model for studying neuronal differentiation on the individual components and on the complete NGC. Differentiated NG108-15 cells grown on recombinant spider silk NWM and collagen fibers formed neuronal networks and synapses. Additionally, whole-cell patch clamp recordings confirmed that all components supported the differentiation of NG108-15 cells into functional neurons. Our NGC demonstrated that tubes made of recombinant spider silk NWM filled with microfluidics-produced collagen fibers are well suited for peripheral nerve repair.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: recombinant spider silk; collagen nerve guidance conduit; neuronal cell differentiation; electrophysiology
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Emerging Fields
Profile Fields > Emerging Fields > Food and Health Sciences
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 20 Jan 2020 12:14
Last Modified: 20 Jan 2020 12:14
URI: https://eref.uni-bayreuth.de/id/eprint/54157