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Analysis of volatile alcohols in apple juices by an electrochemical biosensor measuring in the headspace above the liquid

Title data

Hämmerle, Martin ; Hilgert, Karin ; Horn, Marcus A. ; Moos, Ralf:
Analysis of volatile alcohols in apple juices by an electrochemical biosensor measuring in the headspace above the liquid.
In: Sensors and Actuators B: Chemical. Vol. 158 (2011) Issue 1 . - pp. 313-318.
ISSN 0925-4005
DOI: https://doi.org/10.1016/j.snb.2011.06.026

Official URL: Volltext

Project information

Project title:
Project's official titleProject's id
No informationHA4424/1-3

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

An electrochemical biosensor was optimised for the analysis of volatile alcohols directly from the gas phase without prior absorption or pre-concentration. The sensor is based on the alcohol oxidase (Pichia pastoris) catalyzed conversion of ethanol and the amperometric detection of the generated hydrogen peroxide. Key part of the three-electrode set-up was a gas-diffusion working electrode (potential: +600 mV vs. Ag/AgCl) that consisted of a porous Teflon membrane coated with a thin platinum layer. Headspace samples were analysed for alcohols and used to derive alcohol concentrations in the liquid phase. The biosensor had a sensitivity of 3.43 μA/mM for ethanol, a response time of 69 s, a linear dynamic range of 0.10 – 30 mM, a theoretical detection limit (3 < S/N) of 9.9 μM, and a stability of 86% during continuous operation (18 h @ 1 mM ethanol). Using one sensor on three consecutive days, the mean coefficient of variation was 1.3% (three measurements each day @ 10 mM ethanol). Alcohol contents of three apple juices determined with the biosensor were in the range 0.30 g/l – 0.67 g/l (equivalent to 6.51 mM – 14.5 mM). However, ethanol contents determined by high pressure liquid chromatography coupled to refractive index detection (HPLC-RI) and by a commercial enzyme test kit based on alcohol dehydrogenase ranged from 0.12 g/l to 0.38 g/l (equivalent to 2.60 mM – 8.25 mM). Both indicate that the biosensor detected alcohols other than ethanol in the apple juices. HPLC-RI coupled to the biosensor in a flow-through configuration demonstrated that the biosensor detected methanol concomitant to ethanol. Thus, the biosensor could perform a qualitative analysis of the total content of volatile alcohols in apple juices by analysing the gas phase above the sample. This offers the additional advantage that possible, non-volatile interfering substances in the liquid sample cannot impair the measurement.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER103063
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Functional Materials
Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Research Institutions
Research Institutions > Research Centres
Profile Fields > Advanced Fields > Advanced Materials
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Profile Fields
Profile Fields > Advanced Fields
Result of work at the UBT: Yes
DDC Subjects: 500 Science
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 19 Jan 2015 12:39
Last Modified: 13 Apr 2016 07:20
URI: https://eref.uni-bayreuth.de/id/eprint/5603