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Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution

Title data

Joens, Matthew S. ; Huynh, Chuong ; Kasuboski, James M. ; Ferranti, David ; Sigal, Yury J. ; Zeitvogel, Fabian ; Obst, Martin ; Burkhardt, Claus J. ; Curran, Kevin P. ; Chalasani, Sreekanth H. ; Stern, Lewis A. ; Goetze, Bernhard ; Fitzpatrick, James A. J.:
Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution.
In: Scientific Reports. Vol. 3 (December 2013) . - No. 3514.
ISSN 2045-2322
DOI: https://doi.org/10.1038/srep03514

Abstract in another language

Scanning Electron Microscopy (SEM) has long been the standard in imaging the sub-micrometer surface ultrastructure of both hard and soft materials. In the case of biological samples, it has provided great insights into their physical architecture. However, three of the fundamental challenges in the SEM imaging of soft materials are that of limited imaging resolution at high magnification, charging caused by the insulating properties of most biological samples and the loss of subtle surface features by heavy metal coating. These challenges have recently been overcome with the development of the Helium Ion Microscope (HIM), which boasts advances in charge reduction, minimized sample damage, high surface contrast without the need for metal coating, increased depth of field, and 5 angstrom imaging resolution. We demonstrate the advantages of HIM for imaging biological surfaces as well as compare and contrast the effects of sample preparation techniques and their consequences on sub-nanometer ultrastructure.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER135412
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry > Heisenberg Professorship - Experimental Biogeochemistry - Univ.-Prof. Dr. Martin Obst
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 550 Earth sciences, geology
Date Deposited: 13 Aug 2020 09:04
Last Modified: 15 Sep 2020 08:53
URI: https://eref.uni-bayreuth.de/id/eprint/56472